Changes in myosin heavy chain isoform expression of overloaded rat skeletal muscles.
Changes in myosin heavy chain isoform expression of overloaded rat skeletal muscles.
复制标题
超负荷大鼠骨骼肌肌球蛋白重链亚型表达的变化。
DOI：
10.1016/0020-711x(93)90519-k
复制
发表时间：
1993
期刊：
影响因子：
--
通讯作者：
N. Murakami
中科院分区：
文献类型：
--
作者：
T. Sugiura;H. Miyata;Y. Kawai;H. Matoba;N. Murakami
1.采用十二烷基硫酸钠-丙烯酰胺梯度凝胶电泳法，研究了协同切断腓肠肌产生的功能超负荷对大鼠足底和比目鱼肌肌球蛋白重链(MHC)亚型表达的影响。2.切断腓肠肌5周后，切断腓肠肌诱导的足底肌重44.3%(P<；0.005)，比目鱼肌重37.4%(P<；0.005)。3.在对照和实验足底肌肉中均观察到四种类型的MHC亚型，但在对照和实验肌肉中MHC亚型所占的比例不同，肥厚的足底肌肉中HCI(P<；0.05)、HCIIa和HCIId(P<；0.05)的含量高于对照肌肉，而HCIIb(P<；0.05)的含量低于对照肌肉。4.对照比目鱼肌含有两种MHC亚型：HCI和HCIIa。然而，肥大的比目鱼肌中只有单一的HCI亚型。5.这些结果表明，通过去除增效剂而使骨骼肌超负荷不仅会导致肌肉肥大，而且还会改变MHC亚型的表达。
1. The effect of functional overload produced by tenotomy of synergistic gastrocnemius muscle on the expression of myosin heavy chain (MHC) isoforms in the plantaris and soleus muscles of the rat was studied using gradient sodium dodecyl sulfate-acrylamide gel electrophoresis. 2. Five weeks tenotomy, the plantaris and soleus muscle weights induced by tenotomy of the gastrocnemius muscle were 44.3%(P< 0.005) and 37.4%(P< 0.005), respectively, heavier than the contralateral control muscles. 3. Although four types of MHC isoforms were observed in both control and experimental plantaris, the percentage of MHC isoforms in the control and experimental muscles differed; the hypertrophied plantaris muscle contained more HCI (P< 0.05), HCIIa and HCIId (P< 0.05) and less HCIIb (P< 0.05) than the control muscle. 4. The control soleus muscle contained two MHC isoforms, HCI and HCIIa. However, there was only a single HCI isoform in the hypertrophied soleus muscle. 5. These results indicate that overloading a skeletal muscle by removing its synergists produces not only the muscle hypertrophy but also the changes in the expression of MHC isoforms.
DOI：
10.1016/s0021-9258(18)77444-9
发表时间：
1990-08
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
B. Kirschbaum;H. Kucher;A. Termin;A. Kelly;D. Pette
通讯作者：
B. Kirschbaum;H. Kucher;A. Termin;A. Kelly;D. Pette
成年兔比目鱼肌单纤维的缩短速度与肌球蛋白重链组成相关。
DOI：
--
发表时间：
1985
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Reiser,PJ;Moss,RL;Giulian,GG;Greaser,ML
通讯作者：
Greaser,ML
大鼠骨骼肌2X型肌球蛋白重链的电泳分离及免疫学鉴定
DOI：
10.1016/0304-4165(90)90181-u
发表时间：
1990
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
LaFramboise,WA;Daood,MJ;Guthrie,RD;Moretti,P;Schiaffino,S;Ontell,M
通讯作者：
Ontell,M