Antimicrobial peptide gene induction, involvement of Toll and IMD pathways and defense against bacteria in the red flour beetle, Tribolium castaneum.
Antimicrobial peptide gene induction, involvement of Toll and IMD pathways and defense against bacteria in the red flour beetle, Tribolium castaneum.
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DOI：
10.1016/j.rinim.2012.03.002
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发表时间：
2012
期刊：
影响因子：
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通讯作者：
K. Yokoi;Hiroaki Koyama;C. Minakuchi;Toshiharu Tanaka;K. Miura
中科院分区：
文献类型：
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作者：
K. Yokoi;Hiroaki Koyama;C. Minakuchi;Toshiharu Tanaka;K. Miura
以赤拟谷盗（Tribolium castaneum）为材料，定量研究了活革兰氏阴性菌（大肠杆菌和阴沟肠杆菌）、革兰氏阳性菌（藤黄微球菌和枯草芽孢杆菌）和芽殖酵母（酿酒酵母）对9种抗菌肽（AMP）基因的诱导作用。然后，选择了5个代表性的AMP基因，并研究了Toll和IMD通路在E. coli、M.卢特松群岛利用MyD 88或IMD的RNA干扰技术检测肝细胞癌。结果显示：两种细菌对三种基因的强烈和急性诱导主要由IMD途径介导;两种细菌对一种基因的缓慢和持续诱导主要由Toll途径介导;两种细菌对剩余一种基因的诱导由两种途径介导;酵母对五种基因的诱导由Toll和/或IMD途径介导，这取决于各自的基因。这些结果表明，这两条通路在赤胸果蝇中的激活和使用可能比在黑腹果蝇中更混杂。此外，IMD途径被揭示为主要有助于防御两种细菌物种，革兰氏阴性菌E。泄殖腔和革兰氏阳性B.具有DAP型肽聚糖的枯草杆菌。
UsingTribolium castaneum, we quantitatively investigated the induction of nine antimicrobial peptide (AMP) genes by live gram-negative bacteria (Escherichia coliandEnterobacter cloacae), gram-positive bacteria (Micrococcus luteusandBacillus subtilis) and the budding yeast (Saccharomyces cerevisiae). Then, five representative AMP genes were selected, and the involvement of the Toll and IMD pathways in their induction byE. coli,M. luteusandS. cerevisiaewas examined by utilizing RNA interference of eitherMyD88orIMD. Results indicated: Robust and acute induction of three genes by the two bacterial species was mediated mainly by the IMD pathway; slow and sustained induction of one gene by the two bacteria was mediated mainly by the Toll pathway; induction of the remaining one gene by the two bacteria was mediated by both pathways; induction of the five genes by the yeast was mediated by the Toll and/or IMD pathways depending on respective genes. These results suggest that more promiscuous activation and usage of the two pathways may occur inT. castaneumthan inDrosophila melanogaster. In addition, the IMD pathway was revealed to dominantly contribute to defense against two bacterial species, gram-negativeE. cloacaeand gram-positiveB. subtilisthat possesses DAP-type peptidoglycan.