Bioactive nanoparticles improve calcium handling in failing cardiac myocytes.
Bioactive nanoparticles improve calcium handling in failing cardiac myocytes.
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DOI：
10.2217/nnm.15.126
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发表时间：
2015-11
期刊：
影响因子：
--
通讯作者：
Wagner MB
中科院分区：
文献类型：
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作者：
Maxwell JT;Somasuntharam I;Gray WD;Shen M;Singer JM;Wang B;Saafir T;Crawford BH;Jiang R;Murthy N;Davis ME;Wagner MB
评价N-乙酰葡萄糖胺（GlcNAc）修饰的纳米颗粒及其货物调节衰竭心肌细胞（CM）钙处理的能力。用GlcNAc纳米颗粒处理从正常和衰竭心脏分离的原代CM，以评估纳米颗粒及其货物纠正衰竭肌细胞中功能失调的钙处理的能力。GlcNAc颗粒减少了失败的CM中的异常钙释放并恢复了肌节功能。此外，在GlcNAc纳米颗粒中封装小的钙调节蛋白S100 A1也显示出改善的钙调节。因此，我们的生物活性纳米颗粒的开发允许“两次打击”治疗，通过该治疗，货物和纳米颗粒本身可以调节细胞内蛋白质活性。
To evaluate the ability of N-acetylglucosamine (GlcNAc) decorated nanoparticles and their cargo to modulate calcium handling in failing cardiac myocytes (CMs). Primary CMs isolated from normal and failing hearts were treated with GlcNAc nanoparticles in order to assess the ability of the nanoparticles and their cargo to correct dysfunctional calcium handling in failing myocytes. GlcNAc particles reduced aberrant calcium release in failing CMs and restored sarcomere function. Additionally, encapsulation of a small calcium-modulating protein, S100A1, in GlcNAc nanoparticles also showed improved calcium regulation. Thus, the development of our bioactive nanoparticle allows for a ‘two-hit’ treatment, by which the cargo and also the nanoparticle itself can modulate intracellular protein activity.