Effect of sepsis on mucosal protein synthesis in different parts of the gastrointestinal tract in rats.
Effect of sepsis on mucosal protein synthesis in different parts of the gastrointestinal tract in rats.
复制标题
脓毒症对大鼠胃肠道不同部位粘膜蛋白合成的影响
DOI：
10.1042/cs0870207
复制
发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Hasselgren,PO
中科院分区：
文献类型：
--
作者：
Higashiguchi,T;Noguchi,Y;O'Brien,W;Wagner,K;Fischer,JE;Hasselgren,PO
1.在以前的研究中，我们发现，蛋白质合成率增加了50-60%，在空肠和回肠粘膜脓毒症大鼠。目前尚不清楚脓毒症是否也会影响胃肠道其他部位的蛋白质周转。2.在本研究中，脓毒症对胃肠道不同部位（从胃到直肠）粘膜蛋白质合成的影响在大鼠中进行了测定。3.脓毒症是由盲肠结扎和穿刺;对照组大鼠进行假手术。蛋白质合成率在体内测定后，[14 C]亮氨酸的泛剂量管理。4.结肠的基础粘膜蛋白质合成率低于胃肠道的其他部分。盲肠结扎和穿孔后16小时，小肠、大肠和直肠粘膜的蛋白质合成率增加了40-85%，而胃粘膜的蛋白质合成率则减少了约40%。5.结果表明，粘膜蛋白质的合成速率在胃肠道的各个区域是不同的，并且在胃中对脓毒症的代谢反应与胃肠道的其余部分不同。胃粘膜中蛋白质合成率降低的发现可能部分解释了临床上脓毒症中胃应激性溃疡和出血的倾向。
1. In a previous study we found that the protein synthesis rate was increased by 50-60% in the mucosa of the jejunum and ileum during sepsis in rats. It is not known if sepsis affects protein turnover in other parts of the gastrointestinal tract as well. 2. In the present study, the influence of sepsis on mucosal protein synthesis in different parts of the gastrointestinal tract, from the stomach to the rectum, was determined in rats. 3. Sepsis was induced by caecal ligation and puncture; control rats underwent sham-operation. Protein synthesis rate was measured in vivo after administration of a flooding dose of [14C] leucine. 4. Basal mucosal protein synthesis rates were lower in the colon than in the rest of the gastrointestinal tract. Sixteen hours after caecal ligation and puncture, the protein synthesis rates were increased by 40-85% in the mucosa of the small and large intestine and the rectum, whereas in the gastric mucosa, the protein synthesis rate was reduced by approximately 40%. 5. The results suggest that mucosal protein synthesis rates differ in the various regions of the gastrointestinal tract, and that the metabolic response to sepsis is different in the stomach than in the rest of the gastrointestinal tract. The finding of a reduced protein synthesis rate in the gastric mucosa may partly explain the tendency to gastric stress ulcers and bleeding seen clinically in sepsis.