Glycosylation potential of human prostate cancer cell lines.
Glycosylation potential of human prostate cancer cell lines.
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DOI：
10.1007/s10719-012-9428-8
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发表时间：
2012-10
影响因子：
3
通讯作者：
Brockhausen, Inka
中科院分区：
文献类型：
--
作者：
Gao, Yin;Chachadi, Vishwanath B.;Cheng, Pi-Wan;Brockhausen, Inka
糖基化改变是癌细胞的普遍特征，改变的糖链可以帮助癌细胞逃避免疫监视，促进肿瘤侵袭，增加恶性度。这项研究的目标是识别特定的糖酶，这种糖酶可以区分前列腺癌细胞和正常前列腺细胞。我们研究了几个前列腺细胞中负责组装O-和N-糖链的关键糖基转移酶和磺基转移酶的酶活性和基因表达水平。这些细胞包括来自正常前列腺组织的永生化RWPE-1细胞，以及来自骨转移(PC-3)、脑转移(DU145)、淋巴转移(LNCaP)和脊椎转移(VCaP)的前列腺癌细胞。我们发现，所有的细胞都能够合成具有核心1结构的复杂的N-聚糖和O-聚糖，并且每个细胞系都有独特的生物合成途径来修饰这些结构。体外测定的活性与糖基转移酶和磺基转移酶的mRNA水平有很好的一致性。凝集素和抗体与整个细胞的结合支持这些结果，这形成了开发肿瘤细胞特异性靶向策略的基础。
Altered glycosylation is a universal feature of cancer cells and altered glycans can help cancer cells escape immune surveillance, facilitate tumor invasion, and increase malignancy. The goal of this study was to identify specific glycoenzymes, which could distinguish prostate cancer cells from normal prostatic cells. We investigated enzymatic activities and gene expression levels of key glycosyl- and sulfotransferases responsible for the assembly of O- and N-glycans in several prostatic cells. These cells included immortalized RWPE-1 cells derived from normal prostatic tissues, and prostate cancer cells derived from metastasis in bone (PC-3), brain (DU145), lymph node (LNCaP), and vertebra (VCaP). We found that all cells were capable of synthesizing complex N-glycans and O-glycans with the core 1 structure, and each cell line had characteristic bio-synthetic pathways to modify these structures. The in vitro measured activities corresponded well to the mRNA levels of glycosyltransferases and sulfotransferases. Lectin and antibody binding to whole cells supported these results, which form the basis for the development of tumor cell-specific targeting strategies.
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影响因子：
11.5
作者：
Brown, JR;Fuster, MM;Esko, JD
通讯作者：
Esko, JD
DOI：
10.1111/j.1432-1033.1995.607_2.x
发表时间：
1995-10-15
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
BROCKHAUSEN, I;YANG, JM;TAYLORPAPADIMITRIOU, J
通讯作者：
TAYLORPAPADIMITRIOU, J
影响因子：
2.8
作者：
BELANGER, A;VANHALBEEK, H;LABRIE, F
通讯作者：
LABRIE, F
影响因子：
3
作者：
Gao, Yin;Lazar, Carmen;Brockhausen, Inka
通讯作者：
Brockhausen, Inka
DOI：
10.1016/j.bbagen.2009.05.020
发表时间：
2009-10-01
影响因子：
3
作者：
Brockhausen, Inka;Dowler, Thomas;Paulsen, Hans
通讯作者：
Paulsen, Hans