Differential contribution of steady-state RNA and active transcription in chromatin organization
Differential contribution of steady-state RNA and active transcription in chromatin organization
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DOI：
10.15252/embr.201948068
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发表时间：
2019-08-26
期刊：
影响因子：
7.7
通讯作者：
Rinn, John L.
中科院分区：
文献类型：
--
作者：
Barutcu, A. Rasim;Blencowe, Benjamin J.;Rinn, John L.
核RNA和转录行为与核组织有关。然而，它们对形成高阶染色质组织的基本特征（如拓扑相关结构域（TADs）和基因组区室）的全球贡献仍不清楚。为了研究这些问题，我们进行全基因组染色质构象捕获（Hi-C）分析的存在和不存在的RNase交联前后，或转录抑制剂。核糖核酸酶处理在很大程度上不影响细胞边界，尽管观察到隔室相互作用的微妙破坏。相反，转录抑制导致较弱的边界分数。总的来说，我们的研究结果表明，RNA和转录的相对贡献的差异，以形成广泛使用的Hi-C方法检测到的边界。
Nuclear RNA and the act of transcription have been implicated in nuclear organization. However, their global contribution to shaping fundamental features of higher-order chromatin organization such as topologically associated domains (TADs) and genomic compartments remains unclear. To investigate these questions, we perform genome-wide chromatin conformation capture (Hi-C) analysis in the presence and absence of RNase before and after crosslinking, or a transcriptional inhibitor. TAD boundaries are largely unaffected by RNase treatment, although a subtle disruption of compartmental interactions is observed. In contrast, transcriptional inhibition leads to weaker TAD boundary scores. Collectively, our findings demonstrate differences in the relative contribution of RNA and transcription to the formation of TAD boundaries detected by the widely used Hi-C methodology.