Chlamydia trachomatis utilizes the host cell microtubule network during early events of infection
Chlamydia trachomatis utilizes the host cell microtubule network during early events of infection
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DOI：
10.1046/j.1365-2958.1997.4591832.x
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发表时间：
1997-08-01
影响因子：
3.6
通讯作者：
Birkelund, S
中科院分区：
文献类型：
--
作者：
Clausen, JD;Christiansen, G;Birkelund, S
已知宿主细胞细胞骨架通过提供成功入侵的基础和通过促进病原体一旦进入宿主细胞质内的运动而在几种病原性细胞内微生物的生命周期中起重要作用。用沙眼衣原体血清型E或L2感染的麦考伊细胞，通过间接免疫荧光显微镜观察，发现在从宿主细胞表面迁移到核周位置的过程中，微管和含衣原体的囊泡并置。绝大多数微管相关衣原体囊泡也与酪氨酸磷酸化的麦考伊细胞蛋白搭配。迁移后，含有衣原体的囊泡准确地定位在微管网络的中心，表明衣原体再分布的微管依赖模式，观察到对宿主细胞动力蛋白（已知参与定向囊泡沿沿着运输的微管依赖性运动蛋白）的抑制对C.沙眼的传染性此外，动力蛋白与C.沙眼衣原体E和L2，而不是C。pneumoniae VR-1310，表明C. trachomatis和C.在早期感染事件中发生肺炎。为了支持这一观点，C. pneumoniae VR-1310在摄取过程中诱导的HeLa细胞蛋白酪氨酸磷酸化比C.沙眼
The host cell cytoskeleton is known to play a vital role in the life cycles of several pathogenic intracellular microorganisms by providing the basis for a successful invasion and by promoting movement of the pathogen once inside the host cell cytoplasm. McCoy cells infected with Chlamydia trachomatis serovars E or L2 revealed, by indirect immunofluorescence microscopy, collocation of microtubules and Chlamydia-containing vesicles during the process of migration from the host cell surface to a perinuclear location. The vast majority of microtubule-associated Chlamydia vesicles also collocated with tyrosine-phosphorylated McCoy cell proteins. After migration, the Chlamydia-containing vesicles were positioned exactly at the centre of the microtubule network, indicating a microtubule-dependent mode of chlamydial redistribution, inhibition of host cell dynein, a microtubule-dependent motor protein known to be involved in directed vesicle transport along microtubules, was observed to have a pronounced effect on C. trachomatis infectivity. Furthermore, dynein was found to collocate with perinuclear aggregates of C. trachomatis E and L2 but not C. pneumoniae VR-1310, indicating a marked difference in the cytoskeletal requirements for C. trachomatis and C. pneumoniae during early infection events. In support of this view, C. pneumoniae VR-1310 was shown to induce much less tyrosine phosphorylation of HeLa cell proteins during uptake than that seen for C. trachomatis.