Very low levels of 6-keto-prostaglandin F1 alpha in human plasma.
Very low levels of 6-keto-prostaglandin F1 alpha in human plasma.
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人血浆中 6-酮前列腺素 F1 α 的水平非常低。
DOI：
10.5555/uri:pii:0022214382903006
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
F. Dray
中科院分区：
文献类型：
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作者：
W. Siess;F. Dray
在人血浆和尿液中测定了PGI 2的两种稳定衍生物，即其非酶水解产物（6-keto-PGF 1 α）和酶代谢产物（6，15-diketo-PGF 1 α）。这些化合物在rp-HPLC上分离后通过RIA测定。在rp-HPLC上的样品的先前纯化显著增强了血浆和尿液中这些化合物的RIA测定的特异性。在两种血浆中均检测到PGI 2衍生物6-酮-PGF 1 α（4.7 +/- 3.2 pg/ml，平均值+/- S.D.，n=34）和尿（166 +/-61 pg/ml，n=9）。未发现血浆或尿液中6-keto-PGF 1 α水平的性别差异。血浆或尿液中的PGI 2代谢产物6，15-diketo-PGF 1 α不可测量（分别小于1和小于10 pg/ml，n=4）。当静脉穿刺后立即将[3 H] PGI 2加到柠檬酸盐血液中时，经反相高效液相色谱分析，[3 H] PGI 2完全以[3 H]6-keto-PGF 1 α的形式回收。因此，通过我们的方法，任何循环的PGI 2都将以6-keto-PGF 1 α的形式测量。所获得的结果表明，PGI 2可以在生理条件下存在于人静脉血中，但仅以非常低的浓度存在。（J Lab Clin Med 99：388，1982）
Two stable derivatives of PGI2, its nonenzymatic hydrolysis product (6-keto-PGF1 alpha) and an enzymatic metabolite (6, 15-diketo-PGF1 alpha), were determined in human plasma and urine. These compounds were measured by RIA after separation on rp-HPLC. Previous purification of the samples on rp-HPLC markedly enhanced the specificity of the RIA determinations of those compounds in plasma and urine. The PGI2 derivative 6-keto-PGF1 alpha was detected in both plasma (4.7 +/- 3.2 pg/ml, mean +/- S.D., n=34) and urine (166 +/- 61 pg/ml, n=9). No gender differences of the plasma or urinary levels of 6-keto-PGF1 alpha were found. The PGI2 metabolite 6,15-diketo-PGF1 alpha was not measurable in plasma or urine (less than 1 and less than 10 pg/ml, respectively, n=4). Thus this compound may not be a major endogenous metabolite of PGI2 in man. When [3H]PGI2 was added to citrated blood immediately after venipuncture, it was recovered entirely as [3H]6-keto-PGF1 alpha after rp-HPLC. Therefore any circulating PGI2 would be measured as 6-keto-PGF1 alpha by our method. The results obtained suggest that PGI2 could be present in human venous blood under physiological conditions, but only in very low concentrations. (J Lab Clin Med 99:388, 1982.)