Sesquiterpenes and other constituents of Xylaria sp. NC1214, a fungal endophyte of the moss Hypnum sp.
Sesquiterpenes and other constituents of Xylaria sp. NC1214, a fungal endophyte of the moss Hypnum sp.
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DOI：
10.1016/j.phytochem.2015.08.010
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发表时间：
2015-10
期刊：
影响因子：
3.8
通讯作者：
Gunatilaka AA
中科院分区：
文献类型：
--
作者：
Wei H;Xu YM;Espinosa-Artiles P;Liu MX;Luo JG;U'Ren JM;Arnold AE;Gunatilaka AA
从Xylaria sp. NC1214培养液中分离到氧合的木胍型倍半萜、木胍醇A - d（1-4）、异柴丁烷型倍半萜异柴丁醇A(5)和α-吡啶酮9-羟基木酮(6)，以及5种已知的倍半萜（7-11）和4种已知的细胞松解素（12-15）。所有化合物的结构均通过波谱分析得到，其中1 ~ 5个化合物的相对构型通过NMR noesi实验得到。研究了细胞松弛素C（12）、D（13）和Q（14）对五种肿瘤细胞系的细胞毒活性。细胞松弛素D对5种细胞系均表现出显著的细胞毒性，ic50值范围为0.22 ~ 1.44 μM，而细胞松弛素C和Q表现出中等的选择性细胞毒性。
Oxygenated guaiane-type sesquiterpenes, xylaguaianols A–D (1–4), an iso-cadinane-type sesquiterpene isocadinanol A (5), and an α-pyrone 9-hydroxyxylarone (6), together with five known sesquiterpenes (7–11), and four known cytochalasins (12–15) were isolated from a culture broth of Xylaria sp. NC1214, a fungal endophyte of the moss Hypnum sp. The structures of all compounds were elucidated by the analysis of their spectroscopic data and relative configurations of 1–5 were determined with the help of NMR NOESY experiments. Cytochalasins C (12), D (13), and Q (14) were investigated for their cytotoxic activity against five tumor cell lines. Cytochalasin D showed significant cytotoxicity against all five cell lines, with IC50s ranging from 0.22 to 1.44 μM, whereas cytochalasins C and Q exhibited moderate, but selective cytotoxicity.