Polymerized bonding agents and the differentiation in vitro of human pulp cells into odontoblast-like cells
Polymerized bonding agents and the differentiation in vitro of human pulp cells into odontoblast-like cells
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DOI：
10.1016/j.dental.2004.02.011
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发表时间：
2005-02-01
期刊：
影响因子：
5
通讯作者：
Franquin, JC
中科院分区：
文献类型：
--
作者：
About, I;Camps, J;Franquin, JC
目的：成牙本质细胞是一种高度分化的有丝分裂后细胞，在龋损、牙齿损伤等病理条件下可退化，被其他牙髓细胞替代。我们最近已经证明，这一生理事件可以在体外测试系统中复制，但由于存在未聚合的树脂单体，这种生理事件可以被高度修饰。我们的假设是，键合剂中单体的光聚合可能会消除这些负面影响。本研究的目的是通过牙本质切片观察聚合牙本质粘结剂对成牙本质细胞体外分化的影响。用0.7 mm厚的牙本质片作为粘结剂和培养液之间的屏障，研究四种牙本质粘结剂的粘结效果。将含有粘结剂提取物的培养液以无毒浓度添加到培养细胞上。结果：在此条件下培养的牙髓成纤维细胞合成I型胶原、骨连接蛋白、牙本质唾液蛋白和巢蛋白的水平与对照组相同。此外，牙髓细胞合成了矿化的结节状细胞外基质。这些蛋白在有助于结节形成的细胞中的表达较高。此外，除Nestin外，其余均为。这些蛋白质在矿物结节中表达。意义：这项研究表明，通过牙本质切片，光聚合粘结剂对次生牙本质的细胞分化缺乏影响。(C)2004年牙科材料学会。爱思唯尔有限公司出版。版权所有。
Objectives: Odontoblasts are highly differentiated post-mitotic cells, which under pathological conditions such as carious Lesions and dental injuries may degenerate and be replaced by other pulp cells. We have recently shown that this physiological event can be reproduced in an in vitro assay system, but is highly modified by the presence of unpolymerized resinous monomers. Our hypothesis was that the photopolymerization of the monomers in the bonding agents might abolish these negative effects. The purpose of this study was to evaluate the effects of polymerized dentin bonding agents, through dentin slices, on odontoblast differentiation in vitro.Methods: Pulp cells were obtained from human third molars. They were used to study the effects of four dentin bonding agents through 0.7 mm dentin slices which served as a barrier between the bonding agents and the culture medium. The media containing the bonding agents' extracts were added at non-toxic concentrations onto the cultured cells. Immunohistochemistry was performed to study the differentiation of pulp fibroblasts into odontobtasts under these conditions by evaluating the expression of several odontoblast specific genes.Results: Pulp fibroblasts cultivated under these conditions synthesized type I collagen, osteonectin, dentin sialoprotein and nestin at the same level as in control cultures. Moreover, pulp cells synthesized a mineralized nodular extracellular matrix. Expression of these proteins was higher in the cells contributing to the nodule formation. In addition, except nestin, all. these proteins were expressed in the mineral nodules.Significance: This work shows the lack of effects of photopolymerized bonding agents, through dentin slices, on cytodifferentiation of secondary odontobtasts. (C) 2004 Academy of Dental Materials. Published by Elsevier Ltd. ALL rights reserved.