Possible regulation of FGF activity by syndecan, an integral membrane heparan sulfate proteoglycan.
Possible regulation of FGF activity by syndecan, an integral membrane heparan sulfate proteoglycan.
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Syndecan（一种完整膜硫酸乙酰肝素蛋白多糖）可能调节 FGF 活性。
DOI：
10.1111/j.1749-6632.1991.tb49029.x
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发表时间：
1991
影响因子：
5.2
通讯作者：
Hooper,KC
中科院分区：
文献类型：
--
作者：
Bernfield,M;Hooper,KC
成纤维细胞生长因子（FGF）对肝素具有亲和力（综述见参考文献1、2和3）。这种结合不太可能是一种生理相互作用，因为肝素本身通常不存在于细胞外间隙中（有关详细讨论，请参见参考文献4和5）。肝素蛋白聚糖在细胞内，在肥大细胞和嗜碱性粒细胞的分泌颗粒内，并且当这些细胞在特异性免疫反应位点脱髓鞘时可以变成细胞外的。这些细胞外生长因子与肝素的结合可能代表了与肝素样分子硫酸乙酰肝素的相互作用，硫酸乙酰肝素存在于细胞内、细胞表面和细胞外基质内。许多细胞具有所谓的碱性FGF（bFGF）的低亲和力受体（Kd ca. 2 nM，约每个细胞0.5-2 × 106个结合位点）。这些“受体”具有硫酸乙酰肝素蛋白聚糖的所有特性;结合的FGF可以通过用肝素或硫酸乙酰肝素处理而被置换，并且可以通过用肝素酶或蛋白酶消化而被去除。
The fibroblast growth factors (FGFs) have affinity for heparin (for reviews see References 1, 2, and 3). This binding is not likely a physiological interaction because heparin itself is not usually found in the extracellular space (for extensive discussions, see References 4 and 5). Heparin proteoglycans are intracellular, within the secretory granules of mast cells and basophils, and can become extracellular when these cells degranulate at sites of specific immune reactions. Heparin binding by these extracellular growth factors likely represents interactions with the heparinlike molecule, heparan sulfate, that is found within cells, at the cell surface, and within the extracellular matrix. Many cells possess so-called low-affinity receptors for basic FGF (bFGF)(Kd ca. 2 nM, ca. 0.5-2 x 10 ‘binding sites per cell) on their surfaces.’These “receptors” have all the properties of heparan sulfate proteoglycans; the bound FGF can be displaced by treatment with heparin or heparan sulfate and can be removed by digestion with heparitinase or proteases.’