3D Printing of Cell Culture Devices: Assessment and Prevention of the Cytotoxicity of Photopolymers for Stereolithography
3D Printing of Cell Culture Devices: Assessment and Prevention of the Cytotoxicity of Photopolymers for Stereolithography
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DOI：
10.3390/ma13133011
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发表时间：
2020-07-01
期刊：
影响因子：
3.4
通讯作者：
Egger, Dominik
中科院分区：
文献类型：
--
作者：
Kress, Sebastian;Schaller-Ammann, Roland;Egger, Dominik
3D打印对于先进的、量身定做的细胞培养设备的快速成型越来越重要。在这种背景下，立体光刻代表了一种从光固化聚合物快速生成原型的方法。然而，商业上可获得的光聚合物的生物相容性在很大程度上是未知的。因此，我们评估了六种聚合物的细胞毒性，其中两种根据国际标准化组织10993-5：2009认证为生物相容性，我们评估，如果涂层对二甲苯，一种广泛用于医疗应用的惰性聚合物，可能会保护细胞免受有毒聚合物的细胞毒性影响。此外，我们还对印刷细节的加工性、可靠性和一致性进行了评估。人骨髓间充质干细胞(MSCs)在再生医学中有着广泛的用途和广阔的应用前景，因此被用于细胞毒性检测。将MSCs与打印的光聚合物共同孵育，检测其细胞毒性。所有的光聚合物都显着降低了MSCs的活力，而官方生物相容树脂显示出轻微的毒性作用。此外，涂有对二甲苯的涂层可以完全保护MSCs免受毒性影响。总而言之，没有一种被测试的聚合物可以完全推荐用于细胞培养设备的快速成型。然而，用对二甲苯涂层可以保护细胞免受毒性影响，因此可能是一个可行的选择，直到有更多兼容的材料可用。
3D printing is increasingly important for the rapid prototyping of advanced and tailor-made cell culture devices. In this context, stereolithography represents a method for the rapid generation of prototypes from photocurable polymers. However, the biocompatibility of commercially available photopolymers is largely unknown. Therefore, we evaluated the cytotoxicity of six polymers, two of them certified as biocompatible according to ISO 10993-5:2009, and we evaluated, if coating with Parylene, an inert polymer widely used in medical applications, might shield cells from the cytotoxic effects of a toxic polymer. In addition, we evaluated the processability, reliability, and consistency of the details printed. Human mesenchymal stem cells (MSCs) were used for cytotoxicity testing as they are widely used and promising for numerous applications in regenerative medicine. MSCs were incubated together with printed photopolymers, and the cytotoxicity was assessed. All photopolymers significantly reduced the viability of MSCs while the officially biocompatible resins displayed minor toxic effects. Further, coating with Parylene completely protected MSCs from toxic effects. In conclusion, none of the tested polymers can be fully recommended for rapid prototyping of cell culture devices. However, coating with Parylene can shield cells from toxic effects and thus might represent a viable option until more compatible materials are available.