Fast conventional Fmoc solid-phase peptide synthesis with HCTU
Fast conventional Fmoc solid-phase peptide synthesis with HCTU
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DOI：
10.1002/psc.921
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发表时间：
2008-01-01
影响因子：
2.1
通讯作者：
Park, Jae H.
中科院分区：
文献类型：
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作者：
Hood, Christina A.;Fuentes, German;Park, Jae H.
1H-苯并三唑1-[bis(dimethyl-amino)methylene]-5-chloro-hexafluorophosphate(1-)，3-氧化物(HCTU)是一种无毒、无刺激性、无腐蚀性的偶联剂。以HCTU为偶联剂，合成了7种生物活性多肽(GHRP-6、(65-74)ACP、催产素、G-LHRH、C-肽、hAmylin(1-37)和β-淀粉样蛋白(1-42))，使反应时间缩短到3分钟或更短，偶联时间为5分钟或更短。没有使用昂贵的偶联剂或特殊技术。在不降低粗肽纯度的情况下，总肽合成时间显著缩短42.5h(1.8d)。结果表明，HCTU可以作为一种价廉、高效的偶联剂用于Fmoc固相肽的快速合成。版权所有(C)2007欧洲肽协会和John Wiley&Sons，Ltd.
1H-Benzotriazolium 1-[bis(dimethyl-amino)methylene]-5-chloro-hexafluorophosphate (1-),3-oxide (HCTU) is a nontoxic, nonirritating and noncorrosive coupling reagent. Seven biologically active peptides (GHRP-6, (65-74)ACP, oxytocin, G-LHRH, C-peptide, hAmylin(1-37), and beta-amyloid(1-42)) were synthesized with reaction times reduced to deprotection times of 3 min or less and coupling times of 5 min or less using HCTU as the coupling reagent. Expensive coupling reagents or special techniques were not used. Total peptide synthesis times were dramatically reduced by as much as 42.5 h (1.8 days) without reducing the crude peptide purities. It was shown that HCTU can be used as an affordable, efficient coupling reagent for fast Fmoc solid-phase peptide synthesis. Copyright (C) 2007 European Peptide Society and John Wiley & Sons, Ltd.