An in vitro functionally mature mouse spinal cord preparation for the study of spinal motor networks
An in vitro functionally mature mouse spinal cord preparation for the study of spinal motor networks
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DOI：
10.1016/s0006-8993(98)01199-8
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发表时间：
1999-01-23
期刊：
影响因子：
2.9
通讯作者：
Brownstone, RM
中科院分区：
文献类型：
--
作者：
Jiang, ZY;Carlin, KP;Brownstone, RM
已经在“运动功能成熟”小鼠中开发了体外分离的全脊髓制备物;即发育成熟到足以负重和行走的小鼠。在balb/c小鼠中，这一阶段发生在出生后第10天左右（P10）。士的宁给药引起双侧腰前根的同步活动。节律交替运动样活动可以通过应用5-羟色胺（5-HT），N-甲基-D-天冬氨酸（NMDA）和多巴胺的组合在动物中产生到P12。使用活细胞-死细胞测定法，证明在整个腰髓中主要存在活细胞。下行通路的可行性与胸中部白色物质束的刺激证明。此外，多突触节段性反射可以引起。虽然通常缺席整个脊髓的准备，单突触反射可以总是引起以下纵向中线半切断，导致可能的解释，可能有一个积极的交叉通路产生突触前抑制初级传入终末。这些数据表明，这种功能成熟的脊髓制备可用于研究脊髓生理学，包括运动。(C)1999 Elsevier Science B. V.保留所有权利。
An in vitro isolated whole spinal cord preparation has been developed in 'motor functionally mature' mice; that is mice of developmental maturity sufficient to weight-bear and walk. In balb/c mice this stage occurs at around postnatal day 10 (P10). Administration of strychnine elicited synchronous activity bilaterally in lumbar ventral roots. Rhythmic alternating locomotor-like activity could be produced by application of a combination of serotonin (5-HT), N-methyl-D-aspartate (NMDA), and dopamine in animals up to P12. Using a live cell-dead cell assay, it is demonstrated that there are primarily viable cells throughout the lumbar spinal cord. The viability of descending pathways was demonstrated with stimulation of the mid-thoracic white matter tracts. In addition, polysynaptic segmental reflexes could be elicited. Although usually absent in whole cord preparations, monosynaptic reflexes could invariably be elicited following longitudinal midline hemisection, leading to the possible explanation that there might be an active crossed pathway producing presynaptic inhibition of primary afferent terminals. The data demonstrate that this functionally mature spinal cord preparation can be used for the study of spinal cord physiology including locomotion. (C) 1999 Elsevier Science B.V. All rights reserved.