Anthocyanic vacuolar inclusions (AVIs) selectively bind acylated anthocyanins in Vitis vinifera L. (grapevine) suspension culture
Anthocyanic vacuolar inclusions (AVIs) selectively bind acylated anthocyanins in Vitis vinifera L. (grapevine) suspension culture
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DOI：
10.1023/a:1024028603089
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发表时间：
2003-06-01
影响因子：
2.7
通讯作者：
Franco, C
中科院分区：
文献类型：
--
作者：
Conn, S;Zhang, W;Franco, C
花青素液泡内含物（AVIs）与花青素相互作用，在葡萄细胞悬浮培养液泡中呈现出不同大小的深红色至紫色球体。采用高效液相色谱法（HPLC）对从同一愈伤组织中分离的两株花青素进行了纯化，提取了结合的花青素，并对其进行了分析。将中间色素系（FU-1）与高色素系（FU-2）进行比较，前者酰基化：非酰基化花青素比例为1.3:1，色值为0.84单位，氰化物和牡丹苷为优势种，后者酰基化：非酰基化花青素比例为1.2:1，色值为3.72单位，茉莉苷为优势种。与avi结合的花青素谱显示，两种细胞系中酰基化的花青素增加。28-29%，对花青素类没有明显的偏好。这导致FU-1的酰基化与非酰基化花青素的比例为6.2:1，FU-2为4.9:1。讨论了AVIs对酰化（特别是对香豆素酰化）的选择性优于全细胞的原因。
Anthocyanic vacuolar inclusions (AVIs) appear as dark red-to-purple spheres of various sizes in vacuoles of grapevine (Vitis vinifera L.) cell suspension culture due to their interaction with anthocyanins. AVIs were purified and the bound anthocyanins extracted and analysed by HPLC from two lines of V. vinifera isolated from the same callus accumulating anthocyanin in the dark, yet varying in their anthocyanin profiles and accumulation. An intermediate-pigmented line (FU-1) with a 1.3:1 ratio of acylated: non-acylated anthocyanins, a colour value of 0.84 units and cyanidin and peonidin as the dominant species was compared with a high-pigmented line (FU-2) with a 1.2:1 ratio of acylated: non-acylated anthocyanins, a colour value of 3.72 units and malvidin predominating. The profile of AVI-bound anthocyanins showed an increase in acylated anthocyanins in both lines of approx. 28-29%, with no apparent preference for anthocyanin species. This resulted in a ratio of acylated: non-acylated anthocyanins of 6.2:1 for FU-1 and 4.9:1 for FU-2. The reasons for the selectivity of the AVIs for acylated (specifically p-coumaroylated) species compared with the whole cell profile are discussed.