Increase in gamma interferon-secreting CD8+, as well as CD4+, T cells in lungs following aerosol infection with Mycobacterium tuberculosis
Increase in gamma interferon-secreting CD8+, as well as CD4+, T cells in lungs following aerosol infection with Mycobacterium tuberculosis
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DOI：
10.1128/iai.67.7.3242-3247.1999
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发表时间：
1999-07-01
影响因子：
3.1
通讯作者：
Britton, WJ
中科院分区：
文献类型：
--
作者：
Feng, CG;Bean, AGD;Britton, WJ
虽然已经确定CD 4（+）T细胞是抗结核病（T-B）的保护性免疫应答所必需的，但有一些证据表明，CD 8（+）T细胞也参与了对结核分枝杆菌的宿主应答。然而，关于感染期间肺部CD 8（+）T细胞反应的信息很少。因此，我们比较了结核分枝杆菌气溶胶感染后CD 8（+）和CD 4（+）T细胞的变化。在感染高峰和肺中活化T细胞应答之间观察到延迟。肺中CD 8（+）和CD 4（+）T细胞反应的动力学相同，均在第8周达到峰值，比引流淋巴结中细胞反应的峰值晚4周。在肺CD 8（+）和CD 4（+）T细胞上也发生了类似的激活/记忆表型变化。体外再刺激后，两个子集都合成了γ干扰素，这是一种控制M所必需的细胞因子。肺结核感染。由于肺CD 8（+）T细胞在气溶胶结核分枝杆菌感染期间活跃扩增，因此在未来TB疫苗的设计中靶向CD 8（+）和CD 4（+）T细胞是重要的。
Although it is well established that CD4(+) T cells are required for the protective immune response against tuberculosis (T-B), there is some evidence, that CD8(+) 'T cells are also involved in the host response to Mycobacterium tuberculosis. There is, however, a paucity of information on the pulmonary CD8(+) T-cell response during infection. We therefore have compared the changes in both CD8(+) and CD4(+) T cells following aerosol infection with M tuberculosis. There was an observed delay between the peak of infection and the activated T cell response in the lung. The kinetics of CD8(+) and CD4(+) T-cell responses in the lung were identical, both peaking at week 8, 4 weeks later than the peak of cellular response in draining lymph nodes. Similar changes in activation/memory phenotypes occurred on the pulmonary CD8(+) and CD4(+) T cells. Following in vitro restimulation, both subsets synthesized gamma interferon, a cytokine essential for controlling M. tuberculosis infection. Since lung CD8(+) T cells are actively expanded during aerosol M tuberculosis infection, it is important that both CD8(+) and CD4(+) T cells be targeted in the design of future TB vaccines.