Preconditioning of Human Dental Pulp Stem Cells with Leukocyte- and Platelet-Rich Fibrin-Derived Factors Does Not Enhance Their Neuroregenerative Effect
Preconditioning of Human Dental Pulp Stem Cells with Leukocyte- and Platelet-Rich Fibrin-Derived Factors Does Not Enhance Their Neuroregenerative Effect
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DOI：
10.1155/2019/8589149
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发表时间：
2019-01-01
影响因子：
4.3
通讯作者：
Lambrichts, Ivo
中科院分区：
文献类型：
--
作者：
Gervois, Pascal;Ratajczak, Jessica;Lambrichts, Ivo
中枢神经系统的病理特征是脑组织和神经元功能的丧失，无法通过内源性修复过程充分恢复。这强调需要新的治疗方案，例如能够恢复受损组织或刺激修复的细胞疗法。本研究研究了人牙髓干细胞 (CM-hDPSC) 条件培养基对神经干细胞 (NSC) 增殖和迁移以及初级皮质神经元 (pCN) 神经突生长的神经再生潜力。此外，还评估了富含白细胞和富含血小板的纤维蛋白 (L-PRF) 引发对 NSC 和 pCN 上 hDPSC 分泌组神经再生潜力的影响。 L-PRF 含有增强干细胞诱导再生的因子，但其对 hDPSC 介导的神经再生的影响尚不清楚。这项研究表明 CM-hDPSC 增强了神经突发生。此外，CM-hDPSCs 对 NSCs 具有趋化作用。尽管用 L-PRF 启动 hDPSC 会增加脑源性神经营养因子的分泌，但没有观察到对旁分泌介导的修复机制的额外影响。这些数据支持 hDPSC 的神经再生潜力，尽管启动没有额外的效果，但 L-PRF 启动的 hDPSC 对不同再生机制的潜力仍有待澄清。
Pathologies of the central nervous system are characterized by loss of brain tissue and neuronal function which cannot be adequately restored by endogenous repair processes. This stresses the need for novel treatment options such as cell-based therapies that are able to restore damaged tissue or stimulate repair. This study investigated the neuroregenerative potential of the conditioned medium of human dental pulp stem cells (CM-hDPSCs) on neural stem cell (NSC) proliferation and migration as well as on neurite outgrowth of primary cortical neurons (pCNs). Additionally, the effect of leukocyte- and platelet-rich fibrin (L-PRF) priming on the neuroregenerative potential of the hDPSC secretome on NSCs and pCNs was evaluated. L-PRF contains factors that enhance stem cell-induced regeneration, but its effect on hDPSC-mediated neuroregeneration is unknown. This study demonstrated that CM-hDPSCs enhanced neuritogenesis. Moreover, CM-hDPSCs had a chemoattractant effect on NSCs. Although priming hDPSCs with L-PRF increased brain-derived neurotrophic factor secretion, no additional effects on the paracrine-mediated repair mechanisms were observed. These data support the neuroregenerative potential of hDPSCs, and although priming had no additional effect, the potential of L-PRF-primed hDPSCs on distinct regenerative mechanisms remains to be clarified.