A wireless patch for the monitoring of C-reactive protein in sweat
A wireless patch for the monitoring of C-reactive protein in sweat
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DOI：
10.1038/s41551-023-01059-5
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发表时间：
2023-06
影响因子：
28.1
通讯作者：
J. Tu;Jihong Min;Yu Song;Changhao Xu;Jiahong Li;J. Moore;J. Hanson;Erin Hu;T. Parimon;T. Wang;Elham Davoodi;T. Chou;Peter Chen;J. Hsu;H. Rossiter;W. Gao
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文献类型：
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作者：
J. Tu;Jihong Min;Yu Song;Changhao Xu;Jiahong Li;J. Moore;J. Hanson;Erin Hu;T. Parimon;T. Wang;Elham Davoodi;T. Chou;Peter Chen;J. Hsu;H. Rossiter;W. Gao
血液中蛋白质生物标志物在皮摩尔水平敏感性的定量需要劳动密集型的孵育和洗涤步骤。检测汗液中的蛋白质可以实现即时监测，但它的组成通常存在很大的人际和个人差异，因此受到阻碍。在这里，我们报告了一种可穿戴的无线贴片的设计和性能，用于实时电化学检测汗液中的炎症生物标志物c反应蛋白（CRP）。该设备集成了离子电泳汗液提取，用于汗液采样和试剂路径和更换的微流控通道，以及用于定量CRP的基于石墨烯的传感器阵列（通过抗CRP捕获抗体-共轭金纳米颗粒功能化的电极），离子强度，pH和温度，用于实时校准CRP传感器。在慢性阻塞性肺疾病患者中，有活跃或既往感染或有心力衰竭的患者中，通过贴片测量的CRP浓度升高与血清中蛋白质水平密切相关。用于汗液中炎症蛋白实时敏感分析的可穿戴生物传感器可能有助于慢性疾病的管理。
The quantification of protein biomarkers in blood at picomolar-level sensitivity requires labour-intensive incubation and washing steps. Sensing proteins in sweat, which would allow for point-of-care monitoring, is hindered by the typically large interpersonal and intrapersonal variations in its composition. Here we report the design and performance of a wearable and wireless patch for the real-time electrochemical detection of the inflammatory biomarker C-reactive (CRP) protein in sweat. The device integrates iontophoretic sweat extraction, microfluidic channels for sweat sampling and for reagent routing and replacement, and a graphene-based sensor array for quantifying CRP (via an electrode functionalized with anti-CRP capture antibodies-conjugated gold nanoparticles), ionic strength, pH and temperature for the real-time calibration of the CRP sensor. In patients with chronic obstructive pulmonary disease, with active or past infections or who had heart failure, the elevated concentrations of CRP measured via the patch correlated well with the protein’s levels in serum. Wearable biosensors for the real-time sensitive analysis of inflammatory proteins in sweat may facilitate the management of chronic diseases.