INFLUENCE OF ULTRAVIOLET RADIATION ON THE EXPRESSION OF PROLIFERATING CELL NUCLEAR ANTIGEN AND DNA POLYMERASE α IN SKELETONEMA COSTATUM (BACILLARIOPHYCEAE) 1
INFLUENCE OF ULTRAVIOLET RADIATION ON THE EXPRESSION OF PROLIFERATING CELL NUCLEAR ANTIGEN AND DNA POLYMERASE α IN SKELETONEMA COSTATUM (BACILLARIOPHYCEAE) 1
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DOI：
10.1111/j.1529-8817.2004.03040.x
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发表时间：
2004-08
影响因子：
2.9
通讯作者：
Suzhen Wei;S. L. Hwang;Jeng Chang
中科院分区：
文献类型：
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作者：
Suzhen Wei;S. L. Hwang;Jeng Chang
为了评估紫外线辐射对浮游植物 DNA 复制相关基因表达的影响，采用实时定量 PCR 方法研究了海洋硅藻中肋骨条藻 (Greville) Cleve 的 DNA 聚合酶 α 和增殖细胞核抗原 (PCNA) 的 mRNA 水平。用 UVC 辐射处理藻类培养物 15 分钟会导致处理后 24 小时内严重死亡。与对照实验相比，在处理的培养物中检测到大量胸腺嘧啶二聚体，DNA聚合酶α和PCNA的mRNA水平分别增加了140和23 pmol·(g总RNA)−1。相比之下，接受 UVA/B 辐射的培养物中并未发生大量细胞死亡，胸腺嘧啶二聚体的形成也不明显。此外，UVA/B 不会增强 DNA 聚合酶 α 或 PCNA 的表达水平。根据生物有效紫外线剂量的计算，每天暴露在阳光下可能会使海面中肋沙棘的 DNA 聚合酶 α 或 PCNA 基因的表达量增加 12%。这种增加水平不会严重影响使用这些基因作为生长指标的价值，但将此结论外推到所有浮游植物物种时需要谨慎。
To evaluate the effects of UV radiation on the expression of DNA replication‐related genes in phytoplankton, the mRNA levels of DNA polymerase α and proliferating cell nuclear antigen (PCNA) in a marine diatom, Skeletonema costatum (Greville) Cleve, were studied using the methods of real‐time quantitative PCR. Treating the algal cultures with UVC radiation for 15 min caused severe mortality during the 24‐h period after treatment. A significant amount of thymine dimers was detected in the treated cultures, and the mRNA levels of DNA polymerase α and PCNA increased by as much as 140 and 23 pmol·(g total RNA)−1, respectively, compared with the control experiments. In contrast, massive cell deaths did not occur in cultures receiving UVA/B radiation, and the formation of thymine dimers was inconspicuous. Also, UVA/B did not enhance the expression levels of DNA polymerase α or PCNA. Based on the calculation of biologically effective UV doses, daily exposure to sunlight may increase the expression of DNA polymerase α or PCNA genes in S. costatum by 12% at sea surface. This level of increase does not seriously affect the value of using these genes as growth indicators, but caution is needed in the extrapolation of this conclusion to all phytoplankton species.