Lipid fermentation of corncob residues hydrolysate by oleaginous yeast Trichosporon cutaneum
Lipid fermentation of corncob residues hydrolysate by oleaginous yeast Trichosporon cutaneum
复制标题
产油酵母皮肤毛孢子菌对玉米芯渣水解物的脂质发酵
DOI：
10.1016/j.biortech.2013.11.044
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发表时间：
2014-01-01
影响因子：
11.4
通讯作者：
Bao, Jie
中科院分区：
文献类型：
--
作者：
Gao, Qiuqiang;Cui, Zhenyang;Bao, Jie
玉米芯残渣是指玉米芯经提取木聚糖（半纤维素）生产木糖醇后的纤维素残渣。在此，筛选产油酵母皮状丝孢酵母ACCC 20271用于使用CCR水解物的脂质发酵。以T. cutaneum ACCC 20271.通过改变玉米浆（CSL）的添加量，产生了一个C/N梯度，最佳C/N为49.3。通过改变纤维素酶的添加量，得到不同的初始糖浓度，并随着糖浓度的增加而提高油脂效价。据我们所知，这是第一个报告，使用CCR作为原料的脂肪发酵。在使用木质纤维素生产脂质的研究中，12.3 g/L的脂质滴度和38.4 g/L的细胞干重（DCW）是最高值。(C)2013爱思唯尔有限公司保留所有权利。
Corncob residues (CCR) are cellulose residues of corncob after xylan (hemicellulose) is extracted for production of xylitol. Here, an oleaginous yeast Trichosporon cutaneum ACCC 20271 was screened for lipid fermentation using CCR hydrolysate. The initial carbon-to-nitrogen molar ratio (C/N ratio) and the initial sugar concentration of the CCR hydrolysate were investigated in the lipid fermentation of T. cutaneum ACCC 20271. A C/N ratio gradient was generated by changing the corn steep liquor (CSL) addition and an optimal C/N ratio of 49.3 was obtained. The different initial sugar concentration was obtained by changing the cellulase amount and the lipid titer was enhanced by the increased sugar concentration. To our knowledge, this is the first report on using CCR as the feedstock for lipid fermentation. The lipid titer of 12.3 g/L and dry cell weight (DCW) of 38.4 g/L were the highest values among the studies using lignocellulose for lipid production. (C) 2013 Elsevier Ltd. All rights reserved.