Effect of genetic adjuvants on immune respondance, growth and hormone levels in somatostatin DNA vaccination-induced Hu lambs
Effect of genetic adjuvants on immune respondance, growth and hormone levels in somatostatin DNA vaccination-induced Hu lambs
复制标题
遗传佐剂对生长抑素DNA疫苗接种诱导的湖羔羊免疫反应、生长和激素水平的影响
DOI：
10.1016/j.vaccine.2009.11.058
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发表时间：
2010-02-10
期刊：
影响因子：
5.5
通讯作者：
Yang, Li-Guo
中科院分区：
文献类型：
--
作者：
Xue, Chun-Lin;Liang, Ai-Xin;Yang, Li-Guo
本研究旨在评价生长抑素(SS)和乙肝表面抗原融合基因DNA疫苗(PES/2SS)佐剂的应用前景。将60只雌性HU羔羊随机分为6组，分别以不同佐剂(PE-CpG、大肠杆菌DH5αDNA、粗脂质体或GM-CSF与pES/255联合免疫)进行免疫。对照组为单纯接种PES/2SS组和生理盐水组。大肠杆菌DH5αDNA疫苗组的SS抗体水平明显高于单独接种PES/2SS疫苗组。接种PES/2SS/Pe-CpG或PES/2SS/E.colt DH5α的小鼠体重增加，分别比生理盐水组和仅接种PES/2SS的对照组高33.0%和31.6%。DNA疫苗组GH、IGF-1浓度显著高于生理盐水组，且抗体阳性组高于抗体阴性组。这些结果表明，不同的佐剂/PES/2SS组合可以增强免疫效果，对生长有显著的正效应。(C)2009爱思唯尔有限公司。保留所有权利。
The aim of current study was to evaluate the prospects of adjuvants against DNA vaccination (pES/2SS) encoding somatostatin (SS) and hepatitis B surface antigen fusion gene. A total of 60 female Hu lambs were divided into 6 groups and vaccinated in the context of various adjuvants (and controls): pE-CpG, Escherichia coli DH5 alpha DNA, crude liposomes or GM-CSF in combination with the pES/255 plasmid. Controls included pES/2SS only vaccinated and physiological saline groups. The antibody against SS level in the E. coli DH5 alpha DNA group was significantly increased compared to that in the pES/2SS vaccine alone. Vaccination with pES/2SS/pE-CpG or pES/2SS/E. colt DH5 alpha resulted in elevated weight gains that were 33.0 and 31.6% higher, respectively, than in saline group and pES/2SS only vaccinated controls. The concentrations of GH and IGF-1 in the DNA vaccine groups were remarkably higher than those in the saline group, and those with positive antibody higher than negative antibody. These results suggested that different adjuvant/pES/2SS combinations can enhance the immune effect and had significant positive effects on growth. (C) 2009 Elsevier Ltd. All rights reserved.