Osteoblast-derived cells express functional glucose-dependent insulinotropic peptide receptors.
Osteoblast-derived cells express functional glucose-dependent insulinotropic peptide receptors.
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DOI：
10.1210/endo.141.3.7366
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发表时间：
2000-03
期刊：
影响因子：
4.8
通讯作者：
R. Bollag;Qing Zhong;P. Phillips;L. Min;L. Zhong;Ross Cameron;A. Mulloy;H. Rasmussen;F. Qin;K. Ding;C. Isales
中科院分区：
文献类型：
--
作者：
R. Bollag;Qing Zhong;P. Phillips;L. Min;L. Zhong;Ross Cameron;A. Mulloy;H. Rasmussen;F. Qin;K. Ding;C. Isales
葡萄糖依赖性促胰岛素肽（GIP）是由小肠内分泌细胞合成和分泌的42个氨基酸的肽。GIP在耦合营养摄入和胰岛素分泌中的作用，肠促胰岛素效应，是众所周知的。我们报告说，GIP受体信使RNA和蛋白质存在于正常骨和成骨细胞样细胞系中，并且GIP的高亲和力受体可以通过[125 I]GIP结合研究来证明。当应用于成骨细胞样细胞（SaOS 2）时，GIP刺激细胞内cAMP含量和细胞内钙的增加，两种反应都是剂量依赖性的。此外，GIP的施用导致I型胶原信使RNA的表达升高以及碱性磷酸酶活性的增加。这两种效应都反映了假定成骨细胞的合成代谢作用。这些结果提供了GIP受体存在于骨和成骨细胞样细胞中以及GIP调节这些细胞功能的第一个证据。
Glucose-dependent insulinotropic peptide (GIP) is a 42-amino acid peptide synthesized and secreted from endocrine cells in the small intestine. The role of GIP in coupling nutrient intake and insulin secretion, the incretin effect, is well known. We report that GIP receptor messenger RNA and protein are present in normal bone and osteoblast-like cell lines, and that high affinity receptors for GIP can be demonstrated by [125I]GIP binding studies. When applied to osteoblast-like cells (SaOS2), GIP stimulated increases in cellular cAMP content and intracellular calcium, with both responses being dose dependent. Moreover, administration of GIP results in elevated expression of collagen type I messenger RNA as well as an increase in alkaline phosphatase activity. Both of these effects reflect anabolic actions of presumptive osteoblasts. These results provide the first evidence that GIP receptors are present in bone and osteoblast-like cells and that GIP modulates the function of these cells.