Unilateral nasal obstruction alters sweet taste preference and sweet taste receptors in rat circumvallate papillae
Unilateral nasal obstruction alters sweet taste preference and sweet taste receptors in rat circumvallate papillae
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DOI：
10.1016/j.acthis.2018.10.007
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发表时间：
2019-01-01
影响因子：
2.5
通讯作者：
Ono, Takashi
中科院分区：
文献类型：
--
作者：
Ren, Ershu;Watari, Ippei;Ono, Takashi
鼻塞导致用口呼吸，影响颅面结构的生长发育、口颌系统的肌肉功能以及味觉系统。然而，鼻塞对味觉影响的详细机制尚未完全阐明。在本研究中，我们利用两瓶味觉偏好测试、免疫组织学分析和周乳头中味觉相关分子的 mRNA 表达定量研究了这一机制。新生雄性Wistar大鼠随机分为对照组和实验组。实验组大鼠在8日龄时通过烧灼外鼻孔进行单侧鼻塞。使用项圈夹传感器记录清醒大鼠的动脉氧饱和度（SpO(2)）。评估了五种基本口味解决方案的口味偏好。采用免疫组织化学分析和实时定量聚合酶链反应（RT-PCR）来评估外周乳头味觉细胞中味觉相关分子的表达。在整个实验期间，两组的体重相似。实验组7～12周龄大鼠的SpO(2)显着低于对照组同龄大鼠。在两瓶口味偏好测试中，实验组对甜味的敏感度下降。定量RT-PCR检测结果显示，实验组T1R2、T1R3、α-gustducin和PLC beta 2 mRNA表达量显着低于对照组，并且a-gustducin和PLC beta 2的免疫组化染色不太显着。这些发现表明，鼻塞可能通过大鼠周乳头味觉细胞中味觉相关分子的表达减少而影响甜味感知。
Nasal obstruction causes mouth breathing, and affects the growth and development of craniofacial structures, muscle function in the stomatognathic system, and the taste perceptive system. However, the detailed mechanism underlying the effects of nasal obstruction on taste perception has not been fully elucidated. In this study, we investigated this mechanism using the two-bottle taste preference test, immunohistological analysis, and quantification of the mRNA expression of taste-related molecules in the circumvallate papillae. Neonatal male Wistar rats were divided randomly into control and experimental groups. Rats in the experimental group underwent unilateral nasal obstruction by cauterization of the external nostril at the age of 8 days. Arterial oxygen saturation (SpO(2)) was recorded in awake rats using collar clip sensors. Taste preference for five basic taste solutions was evaluated. Immunohistochemical analysis and quantitative real-time polymerase chain reaction (RT-PCR) were conducted to evaluate the expressions of taste-related molecules in the taste cells of the circumvallate papillae. Body weights were similar between the two groups throughout the experimental period. The SpO(2) in the 7- to 12-week-old rats in the experimental group was significantly lower than that in the age matched rats in the control group. In the two-bottle taste preference test, the sensitivities to sweet taste decreased in the experimental group. The mRNA expression of T1R2, T1R3, alpha-gustducin, and PLC beta 2 was significantly lower in the experimental group than in the control group as determined by quantitative RT-PCR, and the immunohistochemical staining for a-gustducin and PLC beta 2 was less prominent. These findings suggest that nasal obstruction may affect sweet taste perception via the reduced expression of taste-related molecules in the taste cells in rat circumvallate papillae.