The immunostimulatory effect of hot-water extract of Gracilaria tenuistipitata on the white shrimp Litopenaeus vannamei and its resistance against Vibrio alginolyticus
The immunostimulatory effect of hot-water extract of Gracilaria tenuistipitata on the white shrimp Litopenaeus vannamei and its resistance against Vibrio alginolyticus
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DOI：
10.1016/j.fsi.2004.11.009
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发表时间：
2005-08-01
影响因子：
4.7
通讯作者：
Chen, JC
中科院分区：
文献类型：
--
作者：
Hou, WY;Chen, JC
分别用细基江蓠热水提取物4或6 μ g/（-1）注射白色凡纳滨对虾（Litopenaeus vannamei）（10.3 ± 1.5 g），检测其血细胞总数（THC）、酚氧化酶活性、呼吸爆发、超氧化物歧化酶（SOD）活性、吞噬活性和对病原菌溶藻弧菌（Vibrio alginolyticus）的清除效率。L.南美白对虾接种G. tenuistipitata在任何剂量下都显着增加其THC，酚氧化酶活性，呼吸爆发后2天。L.南美白对虾接种G. 6 μ g g（-1）的tenuistipitata在1天后增加了其对溶藻弧菌的吞噬活性和清除效率。在另一个实验中，L.注射G. tenuistipitata用溶藻弧菌以2 × 10（6）菌落形成单位（cfu）虾（-1）攻击，然后置于34份/千份的海水中。用热水浸提液处理的对虾的存活率明显低于对照组。tenuistipitata在6 μ g g（-1）下在3天后以及在实验结束时（攻击后6天）显著高于接受盐水的虾和对照虾。因此，L.南美白对虾接种G. 6 μ g g（-1）或更低的tenuistipitata可提高其免疫能力和对溶藻弧菌感染的抵抗力。(c)2004 Elsevier Ltd.保留所有权利。
The total haemocyte count (THC), phenoloxidase activity, respiratory burst, superoxide dismutase (SOD) activity, phagocytic activity and clearance efficiency to the pathogen Vibrio alginolyticus were examined in the white shrimp Litopenaeus vannamei (10.3 +/- 1.5 g) injected individually with hot-water extract of Gracilaria tenuistipitata at 4 or 6 mu g g(-1). L. vannamei receiving hot-water extract of G. tenuistipitata at either dose increased significantly its THC, phenoloxidase activity, and respiratory burst after 2 days. L. vannamei received hot-water extract of G. tenuistipitata at 6 mu g g(-1) increased its phagocytic activity and clearance efficiency to V. alginolyticus after 1 day. In another experiment, L. vannamei which had been injected with hot-water extract of G. tenuistipitata were challenged with V. alginolyticus at 2 X 10(6) colony-forming units (cfu) shrimp(-1) and then placed in seawater of 34 parts per thousand.. The survival of shrimp that received hot-water extract of G. tenuistipitata at 6 mu g g(-1) was significantly higher than that of shrimp that received saline and the control shrimp after 3 days, and at the termination of the experiment (6 days after the challenge). It is therefore concluded that L. vannamei receiving the hot-water extract of G. tenuistipitata at 6 mu g g(-1) or less increased its immune ability and resistance to V. alginolyticus infection. (c) 2004 Elsevier Ltd. All rights reserved.