Time course of baroreceptor resetting in short-term hypotension in the rat.
Time course of baroreceptor resetting in short-term hypotension in the rat.
复制标题
大鼠短期低血压压力感受器重置的时间过程。
DOI：
10.1152/ajpheart.1978.234.5.h552
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发表时间：
1978
期刊：
影响因子：
--
通讯作者：
E. Krieger
中科院分区：
文献类型：
--
作者：
H. Salgado;E. Krieger
方法雄性和雌性Wistar大鼠，体重200-250 g。在乙醚麻醉下，通过经股动脉插入腹主动脉的塑料套管（PE-10连接到PE-50）测量未麻醉大鼠的直接压力。套管从大鼠背部伸出，并通过30 °连接。cm长的聚乙烯管（PE-90）连接到斯坦森P23-Dd压力传感器，信号从该传感器输入多通道记录仪（Hewlett-Packard型号7858 A）。在压力测量过程中，将大鼠置于小平台（10 x 20 cm和30 cm高）上，在探索性运动的初始阶段后，大鼠保持不受限制且通常安静。尾部体积描记法（15）用于间接测量血压。在戊巴比妥麻醉（30 mg/kg ip）下，在股动脉抽血和输血产生的压力快速变化期间，记录主动脉压力感受器的全神经活动的程序与先前研究中使用的程序相同（16，18，25）。电活动通常记录在孤立的左主动脉降支神经。当压力从低血压水平逐渐变化为严重高血压时，连续记录至少5次压力感受器功能的全范围。注意保持相同的血压变化速度。图1中给出的曲线和压力值说明了这五种测量方法中的一种。结果中给出的所有其他压力值（图图2和3以及表1）是五次测量中最一致的三次测量的平均值。照片上的永久记录-
METHODSMale and female Wistar rats weighing 200-250 g were used. Direct pressure was measured in unanesthetized rats by means of a plastic cannula (PE-10 connected to a PE-50) inserted into the abdominal aorta through the femoral artery under ether anesthesia. The cannula emerged through the back of the rat and was connected through a 30. cm length of polyethylene tubing (PE-90) to a Statham P23-Dd pressure transducer from which signals were fed into a multichannel recorder (Hewlett-Packard model 7858A). During pressure measurements, the rats were placed on a small platform (10 x 20 cm and 30 cm high), where they remained unrestrained and usually quiet after an initial period of exploratory movements. The tail-plethysmographic method (15) was used for indirect measurement of blood pressure. The procedure for recording the whole-nerve activity of the aortic baroreceptor under pentobarbital anesthesia (30 mg/kg ip) during rapid changes in pressure produced by withdrawal and infusion of blood into the femoral artery was the same as that used in previous studies (16, 18, 25). Electrical activity was usually recorded in the isolated left aortic depressor nerve. Full range of baroreceptor function was continuously recorded at least five times when the pressure was progressively changed from low hypotension levels to severe hypertension. Care was taken to maintain the same velocity of blood pressure changes. The tracings and the pressure values given in Fig. 1 illustrate one of these five measurements. All other pressure values given in RESULTS (Figs. 2 and 3 and Table 1) are the averages of the three most consistent of the five measurements. The permanent records made on photo-