Proteomic Changes during MCMV Infection Revealed by iTRAQ Quantitative Proteomic Analysis in Maize
Proteomic Changes during MCMV Infection Revealed by iTRAQ Quantitative Proteomic Analysis in Maize
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iTrAQ 定量蛋白质组分析揭示玉米 mcmv 感染期间的蛋白质组变化
DOI：
10.3390/ijms21010035
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发表时间：
2020-01-01
影响因子：
5.6
通讯作者：
Qian, Yajuan
中科院分区：
文献类型：
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作者：
Dang, Mingqing;Cheng, Qi;Qian, Yajuan
玉米褪绿斑驳病毒（MCMV）是一种严重危害玉米产量的病毒。为了更好地研究MCMV感染对玉米植物的破坏作用，对MCMV感染的玉米品种进行基于同量异位素标记的相对和绝对定量（iTRAQ）比较蛋白质组学分析。B73共有972个差异丰富的蛋白质（DAP），包括661个蛋白质的丰度增加和311个蛋白质的丰度降低，被确定为响应MCMV感染。DAP的功能注释和光合活性的测量表明，光合作用下降，而核糖体蛋白，蛋白质的丰度与胁迫反应，氧化还原和氧化还原稳态的MCMV感染期间显着改变。通过黄瓜花叶病毒诱导的基因沉默（CMV-VIGS），进一步分析了二硫键异构酶样蛋白ZmPDIL-1和过氧化物氧还蛋白家族蛋白ZmPrx 5在MCMV感染过程中的作用。在ZmPDIL-1和ZmPrx 5沉默的B73玉米中MCMV的积累受到抑制，表明ZmPDIL-1和ZmPrx 5可能增强宿主对MCMV感染的易感性。
Maize chlorotic mottle virus (MCMV) has been occurring frequently worldwide and causes severe yield losses in maize (Zea mays). To better investigate the destructive effects of MCMV infection on maize plants, isobaric tagging for relative and absolute quantitation (iTRAQ)-based comparative proteomic analysis was performed on MCMV infected maize cv. B73. A total of 972 differentially abundant proteins (DAPs), including 661 proteins with increased abundance and 311 proteins with reduced abundance, were identified in response to MCMV infection. Functional annotations of DAPs and measurement of photosynthetic activity revealed that photosynthesis was decreased, while the abundance of ribosomal proteins, proteins related to stress responses, oxidation-reduction and redox homeostasis was altered significantly during MCMV infection. Two DAPs, disulfide isomerases like protein ZmPDIL-1 and peroxiredoxin family protein ZmPrx5, were further analyzed for their roles during MCMV infection through cucumber mosaic virus-based virus-induced gene silencing (CMV-VIGS). The accumulation of MCMV was suppressed in ZmPDIL-1-silenced or ZmPrx5-silenced B73 maize, suggesting ZmPDIL-1 and ZmPrx5 might enhance host susceptibility to MCMV infection.