Adult human keratinocytes migrating over nonviable dermal collagen produce collagenolytic enzymes that degrade type I and type IV collagen.
Adult human keratinocytes migrating over nonviable dermal collagen produce collagenolytic enzymes that degrade type I and type IV collagen.
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成人角质形成细胞在非活性真皮胶原蛋白上迁移，产生胶原蛋白分解酶，可降解 I 型和 IV 型胶原蛋白。
DOI：
10.1111/1523-1747.ep12285689
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Liotta,L
中科院分区：
文献类型：
--
作者：
Woodley,DT;Kalebec,T;Banes,AJ;Link,W;Prunieras,M;Liotta,L
在无成纤维细胞的培养中，成人角质形成细胞迁移到不能存活的真皮基质上，会导致迁移上皮下的胶原基质变薄和降解。此外，培养物中的非浓缩条件培养液对I型和IV型胶原均显示出胶原酶活性，EDTA可抑制该活性，而苯甲基磺酰氟或N-乙基马来酰亚胺则不抑制该活性。由于迁移上皮和真皮基质不含成纤维细胞，本研究表明，迁移角质形成细胞与间质胶原接触时能够产生抗I型和IV型胶原的胶原酶。此外，迁移性角质形成细胞在降解基底膜胶原的能力上似乎与高转移细胞相似。
Human adult keratinocytes migrating on a nonviable dermal substrate in cultures without fibroblasts induce thinning and degradation of the collagen substrate beneath the migrating epithelium. Further, unconcentrated conditioned medium from the cultures exhibit collagenolytic activity against both type I and type IV collagen which is inhibited by EDTA but not by phenylmethylsulfonyl fluoride or N-ethylmaleimide. Since the migrating epithelium and dermal substrate do not contain fibroblasts, this study shows that migratory keratinocytes in contact with interstitial collagen are capable of producing collagenases against type I and type IV collagen. Moreover, migratory keratinocytes appear to be similar to highly metastatic cells in their ability to degrade basement membrane collagen.