Nile Tilapia: A Model for Studying Teleost Color Patterns
Nile Tilapia: A Model for Studying Teleost Color Patterns
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尼罗罗非鱼：研究硬骨鱼颜色模式的模型
DOI：
10.1093/jhered/esab018
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发表时间：
2021
影响因子：
3.1
通讯作者：
Wang Deshou
中科院分区：
文献类型：
--
作者：
Wang Chenxu;Lu Baoyue;Li Tao;Liang Guangyuan;Xu Mengmeng;Liu Xingyong;Tao Wenjing;Zhou Linyan;Kocher Thomas D.;Wang Deshou
慈鲷科鱼类多样的颜色模式在配偶选择和物种形成中起着重要的作用。在这里，我们开发了尼罗罗非鱼（Oreochromis niloticus）作为模式系统研究的慈鲷鱼的颜色模式的发育遗传学。我们确定了4种类型的色素细胞：黑色素细胞，黄色素细胞，虹膜细胞和红色素细胞，其特点是在野生型鱼的第一次出现。我们突变了25个参与黑素生成、蝶啶代谢、类胡萝卜素吸收和裂解途径的基因。在25个突变基因中，有13个基因在F0和F2代都具有表型。F1杂合子均无表型。通过比较我们的突变体的颜色模式与红罗非鱼（Oreochromis spp），一个自然的突变体，在罗非鱼物种的杂交过程中产生的，我们发现，身体和眼睛的色素是由不同的基因控制。先前研究的基因如emitf、kita/kitlga、pmel、tyrb、hps 4、gch 2、csf 1 ra、pax 7 b和bco 2b被证明对罗非鱼的颜色模式具有重要意义。我们的研究结果表明，罗非鱼，一种具有4种类型的色素细胞和垂直条纹野生型的颜色模式，以及各种天然和人工诱导的颜色基因突变体，可以作为一个很好的模型系统，研究颜色图案的脊椎动物。
The diverse color patterns of cichlid fishes play an important role in mate choice and speciation. Here we develop the Nile tilapia (Oreochromis niloticus) as a model system for studying the developmental genetics of cichlid color patterns. We identified 4 types of pigment cells: melanophores, xanthophores, iridophores and erythrophores, and characterized their first appearance in wild-type fish. We mutated 25 genes involved in melanogenesis, pteridine metabolism, and the carotenoid absorption and cleavage pathways. Among the 25 mutated genes, 13 genes had a phenotype in both the F0 and F2 generations. None of F1 heterozygotes had phenotype. By comparing the color pattern of our mutants with that of red tilapia (Oreochromis spp), a natural mutant produced during hybridization of tilapia species, we found that the pigmentation of the body and eye is controlled by different genes. Previously studied genes likemitf,kita/kitlga,pmel,tyrb,hps4,gch2,csf1ra,pax7b,andbco2bwere proved to be of great significance for color patterning in tilapia. Our results suggested that tilapia, a fish with 4 types of pigment cells and a vertically barred wild-type color pattern, together with various natural and artificially induced color gene mutants, can serve as an excellent model system for study color patterning in vertebrates.