Nonvolatile copolymer compositions for fabricating gel element microarrays.
Nonvolatile copolymer compositions for fabricating gel element microarrays.
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用于制造凝胶元件微阵列的非挥发性共聚物组合物。
DOI：
10.1016/j.ab.2011.09.028
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发表时间：
2012
影响因子：
2.9
通讯作者：
Chandler,DarrellP
中科院分区：
文献类型：
--
作者：
Golova,JuliaB;Chernov,BorisK;Perov,AlexanderN;Reynolds,Jennifer;Linger,YvonneL;Kukhtin,Alexander;Chandler,DarrellP
By modifying polymer compositions and cross-linking reagents, we have developed a simple yet effective manufacturing strategy for copolymerized three-dimensional gel element arrays. A new gel-forming monomer, 2-(hydroxyethyl) methacrylamide (HEMAA), was used. HEMAA possesses low volatility and improves the stability of copolymerized gel element arrays to on-chip thermal cycling procedures relative to previously used monomers. Probe immobilization efficiency within the new polymer was 55%, equivalent to that obtained with acrylamide (AA) and methacrylamide (MA) monomers. Nonspecific binding of single-stranded targets was equivalent for all monomers. Increasing cross-linker chain length improved hybridization kinetics and end-point signal intensities relative to N,N-methylenebisacrylamide (Bis). The new copolymer formulation was successfully applied to a model orthopox array. Because HEMAA greatly simplifies gel element array manufacture, we expect it (in combination with new cross-linkers described here) to find widespread application in microarray science.
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