Immunogenicity and antigenicity of Plasmodium vivax merozoite surface protein 10
Immunogenicity and antigenicity of Plasmodium vivax merozoite surface protein 10
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DOI：
10.1007/s00436-014-3907-8
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发表时间：
2014-07-01
影响因子：
2
通讯作者：
Han, Eun-Taek
中科院分区：
文献类型：
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作者：
Cheng, Yang;Wang, Bo;Han, Eun-Taek
在参与裂殖子入侵的蛋白质中，糖基磷脂酰肌醇锚定蛋白（GPI-AP）被认为是潜在的疫苗候选者，因为它们定位于顶端细胞器和表面;这些候选者被预测在入侵过程中发挥重要作用。间日疟原虫裂殖子表面蛋白10（Plasmodium vivax merozoite surface protein 10，PvMSP-10）是一种GPI-AP。然而，在之前的一项研究中，如此高的抗体滴度对受到间日疟原虫寄生虫攻击的动物没有显示出保护作用。为了充分评估免疫原性并进一步表征PvMSP-10以了解其疫苗潜力，我们通过用无细胞表达的重组PvMSP-10蛋白免疫BALB/c小鼠来评估其免疫原性。MSP-10的抗原性进行了分析，我们发现42%的灵敏度和95%的特异性使用血清样品从P. vivax感染的韩国患者。IgG 1和IgG 3是间日疟患者血清中抗PvMSP-10的主要免疫反应性抗体，并且IgG 1和IgG 3以及Th 1型细胞因子在PvMSP-10免疫的小鼠中主要分泌。MSP-10具有良好的免疫原性和抗原性，可作为间日疟疫苗。
Among the proteins involved in the invasion by merozoite, the glycosylphosphatidylinositol-anchored proteins (GPI-APs) are suggested as potential vaccine candidates because of their localization to apical organelles and the surface; these candidates are predicted to play essential roles during invasion. As a GPI-AP, Plasmodium vivax merozoite surface protein 10 (PvMSP-10) induces high antibody titers. However, such high antibody titers have shown no protective efficacy for animals challenged with P. vivax parasites in a previous study. To adequately evaluate the immunogenicity and further characterize PvMSP-10 in order to understand its vaccine potential, we assessed its immunogenicity by immunizing BALB/c mice with cell-free expressed recombinant PvMSP-10 protein. The antigenicity of MSP-10 was analyzed, and we found 42 % sensitivity and 95 % specificity using serum samples from P. vivax-infected Korean patients. The IgG1 and IgG3 were the predominant immunoreactive antibodies against PvMSP-10 in vivax patient sera, and IgG1 and IgG3 and Th1-type cytokines were predominantly secreted in PvMSP-10-immunized mice. We conclude that the immunogenicity and antigenicity of MSP-10 may serve as a potential vaccine against vivax malaria.