Multi-compartment encapsulation of communicating droplets and droplet networks in hydrogel as a model for artificial cells.
Multi-compartment encapsulation of communicating droplets and droplet networks in hydrogel as a model for artificial cells.
复制标题
DOI：
10.1038/srep45167
复制
发表时间：
2017-04-03
影响因子：
4.6
通讯作者：
Sapra KT
中科院分区：
文献类型：
--
作者：
Bayoumi M;Bayley H;Maglia G;Sapra KT
构建细胞模拟物是合成生物学家面临的主要挑战。为此目的的努力主要集中在脂质和聚合物封装的容器，脂质体和聚合物囊泡，分别。在这里，我们介绍了一个多室，嵌套系统，包括稳定在油/脂质混合物，所有封装在水凝胶中的水滴。功能性能力（电和化学通信）通过跨越在包封的水性液滴和包封的水凝胶的界面处形成的脂质双层的蛋白质纳米孔赋予。至关重要的是，区室化使得能够以受控的方式形成两个相邻的脂质双层，这是实现功能性原始细胞或原始组织的要求。
Constructing a cell mimic is a major challenge posed by synthetic biologists. Efforts to this end have been primarily focused on lipid- and polymer-encapsulated containers, liposomes and polymersomes, respectively. Here, we introduce a multi-compartment, nested system comprising aqueous droplets stabilized in an oil/lipid mixture, all encapsulated in hydrogel. Functional capabilities (electrical and chemical communication) were imparted by protein nanopores spanning the lipid bilayer formed at the interface of the encapsulated aqueous droplets and the encasing hydrogel. Crucially, the compartmentalization enabled the formation of two adjoining lipid bilayers in a controlled manner, a requirement for the realization of a functional protocell or prototissue.