Genotypic Diversity and Epidemiology of Human Rhinovirus Among Children With Severe Acute Respiratory Tract Infection in Shanghai, 2013-2015
Genotypic Diversity and Epidemiology of Human Rhinovirus Among Children With Severe Acute Respiratory Tract Infection in Shanghai, 2013-2015
复制标题
DOI：
10.3389/fmicb.2018.01836
复制
发表时间：
2018-08-07
影响因子：
5.2
通讯作者：
Tan, Wenjie
中科院分区：
文献类型：
--
作者：
Zhao, Yanjie;Shen, Jun;Tan, Wenjie
人鼻病毒（HRV），特别是HRV-C，越来越多地被认为是严重急性呼吸道感染（SARI）的原因。然而，对SARI患儿HRV的基因型多样性和流行病学知之甚少。因此，我们调查了中国SARI儿童2年内HRV的基因型多样性和流行病学。从2013年至2015年在上海因SARI住院的儿童中收集了总计1，003份鼻咽抽吸物。通过靶向病毒5' UTR的PCR方法筛选HRV，并通过对HRV基因组的VP 4-VP 2区域进行测序进行基因分型。我们还筛查了15种其他常见的呼吸道病毒，以评估与HRV合并感染的患病率。对患者人口统计学和临床数据进行了审查。在1，003份标本中，280份（27.9%）检出HRV：140份（14.0%）为HRV-A，21份（2.1%）为HRV-B，56份（5.6%）为HRV-C，63份（6.3%）为HRV-未分型。系统发育分析共鉴定出77种基因型（HRV-A型43种，HRV-B型10种，HRV-C型24种），其中以A78、A12、A89、B70、C2、C6和C24为主。HRV-A主要在2013年冬季和2014年秋季检测到，而HRV-C检测在2013年秋季和2014年秋季达到高峰。HRV-A的检出率在患者中最高
Human rhinovirus (HRV), and particularly HRV-C, is increasingly recognized as a cause of severe acute respiratory infections (SARIs). However, little is known about the genotypic diversity and epidemiology of HRV among children with SARI. Thus, we investigated the genotypic diversity and epidemiology of HRV in children with SARI in China over a 2-year period. In total 1,003, nasopharyngeal aspirates were collected from children hospitalized with SARI in Shanghai from 2013 to 2015. HRV was screened for by a PCR method targeting the viral 5' UTR and was genotyped by sequencing of the VP4-VP2 region of the HRV genome. We also screened for 15 other common respiratory viruses to assess the prevalence of co-infection with HRV. The patient demographic and clinical data were reviewed. HRV was detected in 280 (27.9%) of the 1,003 specimens: HRV-A in 140 (14.0%), HRV-B in 21 (2.1 %), HRV-C in 56 (5.6%), and HRV-untyped in 63 (6.3%). A phylogenetic analysis identified 77 genotypes (43 HRV-A, 10 HRV-B, and 24 HRV-C), among which A78, A12, A89, B70, C2, C6, and C24 predominated. HRV-A was detected mainly in winter 2013 and autumn 2014, while HRV-C detection peaked in autumn 2013 and 2014. The detection frequency of HRV-A was highest in patients