Fabrication of Nerve Growth Factor Encapsulated Aligned Poly(ε-Caprolactone) Nanofibers and Their Assessment as a Potential Neural Tissue Engineering Scaffold
Fabrication of Nerve Growth Factor Encapsulated Aligned Poly(ε-Caprolactone) Nanofibers and Their Assessment as a Potential Neural Tissue Engineering Scaffold
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DOI：
10.3390/polym8020054
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发表时间：
2016-02-01
期刊：
影响因子：
5
通讯作者：
Ramakrishna, Seeram
中科院分区：
文献类型：
--
作者：
Hu, Jue;Tian, Lingling;Ramakrishna, Seeram
周围神经损伤是一个亟待解决的临床问题。神经组织工程为促进周围神经损伤的再生提供了一条很有前途的途径。本研究采用乳液静电纺丝技术制备了无规（R）和定向（A）聚己内酯（PCL）-神经生长因子（NGF）&牛血清白蛋白（BSA）纳米纤维支架[（R/A）-PCL-NGF&BSA]，其中NGF和BSA包封在核中，PCL形成壳。随机和对齐的纯PCL，PCL-BSA，和PCL-NGF纳米纤维也被生产用于比较。通过场发射扫描电镜（FESEM）和水接触角测试对支架进行了表征。释放研究表明，与稳定剂BSA的加入，从乳液静电纺丝PCL纳米纤维的持续释放的NGF观察超过28天。[3-(4 MTS]分析显示（R/A）-PCL-NGF和（R/A）-PCL-NGF&BSA支架有利于细胞生长，并且对PC 12细胞没有显示出细胞毒性。激光扫描共聚焦显微镜图像显示A-PCL-NGF&BSA支架增加了神经突起的长度，并引导神经突起沿纤维轴沿着延伸，表明A-PCL-NGF&BSA支架具有引导神经组织生长和促进神经再生的潜力。
Peripheral nerve injury is a serious clinical problem to be solved. There has been no breakthrough so far and neural tissue engineering offers a promising approach to promote the regeneration of peripheral neural injuries. In this study, emulsion electrospinning technique was introduced as a flexible and promising technique for the fabrication of random (R) and aligned (A) Poly(epsilon-caprolactone) (PCL)-Nerve Growth Factor (NGF)&Bovine Serum Albumin (BSA) nanofibrous scaffolds [(R/A)-PCL-NGF&BSA], where NGF and BSA were encapsulated in the core while PCL form the shell. Random and aligned pure PCL, PCL-BSA, and PCL-NGF nanofibers were also produced for comparison. The scaffolds were characterized by Field Emission Scanning Electron Microscopy (FESEM) and water contact angle test. Release study showed that, with the addition of stabilizer BSA, a sustained release of NGF from emulsion electrospun PCL nanofibers was observed over 28 days. [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt; MTS] assay revealed that (R/A)-PCL-NGF and (R/A)-PCL-NGF&BSA scaffolds favored cell growth and showed no cytotoxicity to PC12 cells. Laser scanning confocal microscope images exhibited that the A-PCL-NGF&BSA scaffold increased the length of neurites and directed neurites extension along the fiber axis, indicating that the A-PCL-NGF&BSA scaffold has a potential for guiding nerve tissue growth and promoting nerve regeneration.