Characterization of the receptors for chicken GHRH and GHRH-related peptides: Identification of a novel receptor for GHRH and the receptor for GHRH-LP (PRP)
Characterization of the receptors for chicken GHRH and GHRH-related peptides: Identification of a novel receptor for GHRH and the receptor for GHRH-LP (PRP)
复制标题
鸡 GHRH 和 GHRH 相关肽受体的表征：新型 GHRH 受体和 GHRH-LP (PRP) 受体的鉴定
DOI：
10.1016/j.domaniend.2009.07.008
复制
发表时间：
2010-01-01
影响因子：
2.1
通讯作者：
Leung, F. C.
中科院分区：
文献类型：
--
作者：
Wang, Y.;Li, J.;Leung, F. C.
生长激素释放激素及其结构相关肽，GHRH样肽（GHRH-LP）（也称为PRP）、肽组氨酸异亮氨酸（PHI）、血管活性肠肽（VIP）和垂体腺苷酸环化酶激活肽（PACAP）已被报道在脊椎动物的垂体和垂体外组织中发挥重要的生理作用；然而，人们对鸟类中这些 GHRH 相关肽受体的身份知之甚少。在这项研究中，从鸡脑或垂体中克隆了6种GHRH受体和GHRH相关肽（cGHRHR（1），cGHRHR（2），cGHRH-LPR，cPAC（1），cVPAC（1）和cVPAC（2）），并使用pGL3-CHOCRE-荧光素酶报告系统在中国仓鼠卵巢（）细胞中检查了它们的功能。结果表明：（一）所有受体均为与细胞内PKA信号通路功能偶联的G蛋白偶联受体； (2)鉴定出2个GHRH受体(cGHRHR(1)和cGHRHR(2))，并且这两种受体均可被cGHRH有效激活； (3)cGHRH-LP可激活其特异性受体cGHRH-LPR(cPRP-R)，同时也激活cGHRHR(1)和cGHRHR(2)； (4) PACAP可有效激活其受体cPAC(1)、cVPAC(1)和cVPAC(2)；然而，cVPAC(1)和cVPAC(2)也可以被cVIP和tPHI有效激活，表明它们可以作为VIP受体和潜在的PHI受体。使用逆转录聚合酶链反应测定，我们进一步检查了成年鸡组织中这些受体的 mRNA 表达。 cGHRHR(1)、cGHRHR(2)和cGHRH-LPR的表达主要限于垂体和/或脑，而cPAC(1)、cVPAC(1)和cVPAC(2)在大多数检查的组织中表达。总的来说，我们的研究鉴定了鸡 GHRH 受体和 GHRH 相关肽，包括一种新型 GHRH 受体 (cGHRHR(2))，并为阐明这些肽在靶组织中的作用奠定了基础。 (C) 2009 Elsevier Inc. 保留所有权利。
Growth hormone releasing hormone and its structurally related peptides, GHRH-like peptide (GHRH-LP) (also called PRP), peptide histidine-isoleucine (PHI), vasoactive intestinal polypeptide (VIP), and pituitary adenylate cyclase-activating polypeptide (PACAP), have been reported to play important physiological roles in pituitary and extrapituitary tissues of vertebrates; however, little is known about the identity of these GHRH-related peptide receptors in birds. In this study, 6 receptors for GHRH and GHRH-related peptides (cGHRHR(1), cGHRHR(2), cGHRH-LPR, cPAC(1), cVPAC(1), and cVPAC(2)) were cloned from chicken brain or pituitary, and their functionalities were examined in Chinese hamster ovary (CHO) cells using a pGL3-CRE-luciferase reporter system. Results showed that: (I) all receptors are G protein coupled receptors functionally coupled to the intracellular PKA signaling pathway; (2) 2 GHRH receptors (cGHRHR(1) and cGHRHR(2)) were identified, and both receptors could be potently activated by cGHRH; (3) cGHRH-LP could activate its specific receptor cGHRH-LPR (cPRP-R), and it also activated cGHRHR(1) and cGHRHR(2); and (4) PACAP could potently activate its receptors cPAC(1), cVPAC(1) and cVPAC(2); however, cVPAC(1) and cVPAC(2) could also be effectively activated by cVIP and tPHI, indicating that they can serve as VIP receptors and potential PHI receptors. Using a reverse transcription polymerase chain reaction assay, we further examined the mRNA expression of these receptors in adult chicken tissues. The expressions of cGHRHR(1), cGHRHR(2), and cGHRH-LPR are restricted mainly to the pituitary and/or brain, whereas cPAC(1), cVPAC(1), and cVPAC(2) are expressed in most of the tissues examined. Collectively, our study identified the receptors for chicken GHRH and GHRH-related peptides, including a novel GHRH receptor (cGHRHR(2)), and established a basis to elucidate the roles of these peptides in target tissues. (C) 2009 Elsevier Inc. All rights reserved.