Rhesus D Antigenic Determinants on Residual Red Blood Cells in Apheresis and Buffy Coat Platelet Concentrates
Rhesus D Antigenic Determinants on Residual Red Blood Cells in Apheresis and Buffy Coat Platelet Concentrates
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单采和血沉棕黄层血小板浓缩液中残留红细胞的恒河猴 D 抗原决定因素
DOI：
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发表时间：
2019
影响因子：
2.2
通讯作者：
A. Lebrun
中科院分区：
文献类型：
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作者：
L. Thibault;M. D. de Grandmont;M. Cayer;N. Dussault;A. Jacques;É. Ducas;A. Beauséjour;A. Lebrun
背景资料：血小板浓缩物（PC）中残留红细胞（RBC）的水平是令人感兴趣的，因为输血患者中RBC抗原的同种异体免疫相关的临床问题。这项工作的目的是表征和定量的水平的完整和破碎的红细胞在单采（AP-PC）和血沉棕黄层PC（BC-PC），以评估其潜在的风险RhD抗原同种异体免疫。研究方法：在用抗CD 41（血小板）和抗CD 235 a（RBC）抗体染色后，通过流式细胞术分析完整和片段化RBC上RhD抗原的大小和密度。结果如下：AP-PC中残留RBC计数为29 ± 22 × 106/单位，BC-PC中为121 ± 54 × 106/单位，分别相当于约3和11 µL RBC副产物。在AP-PC中，RBC颗粒上的RhD表达高约4倍，这些颗粒分别占BC-PC和AP-PC中总抗原负荷的66%和75%。结论：处理方法影响PC中污染残留RBC和RBC衍生颗粒的数量和性质。这些血液制品中残留RBC的估计通常基于完整RBC的测量，这可能低估了输血患者中同种免疫的风险。这些RBC衍生的颗粒是否可以产生免疫应答，因此是否应该考虑用于Rh免疫预防性治疗的问题仍有待澄清。
Background: The level of residual red blood cells (RBCs) in platelet concentrates (PCs) is of interest because of clinical concerns related to alloimmunization to RBC antigens in transfused patients. This work aims at characterizing and quantifying the levels of intact and fragmented RBCs in apheresis (AP-PCs) and buffy coat PCs (BC-PCs) to assess their potential risk for RhD antigen alloimmunization. Methods: After staining with anti-CD41 (platelets) and anti-CD235a (RBCs) antibodies, the size and density of RhD antigen on intact and fragmented RBCs were analyzed by flow cytometry. Results: Residual RBC counts were 29 ± 22 × 106/unit in AP-PCs and 121 ± 54 × 106/unit in BC-PCs, which correspond to about 3 and 11 µL of RBCs by product, respectively. RhD expression was about 4 times higher on RBC particles in AP-PCs, and these particles contribute to 66 and 75% of the total antigenic load in BC-PCs and AP-PCs, respectively. Conclusions: Processing methods influence the quantity and nature of contaminating residual RBCs and RBC-derived particles in PCs. The estimation of residual RBCs in these blood products is generally based on measurements of intact RBCs, which might underestimate the risk for alloimmunization in transfused patients. The question of whether these RBC-derived particles can produce an immune response and, thus, should then be taken into consideration for Rh immune prophylactic treatments, remains to be clarified.
影响因子：
3.6
作者：
Westhoff, Connie M.
通讯作者：
Westhoff, Connie M.