Genetic diversity of oilseed Brassica napus inbred lines based on sequence-related amplified polymorphism and its relation to hybrid performance
Genetic diversity of oilseed Brassica napus inbred lines based on sequence-related amplified polymorphism and its relation to hybrid performance
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DOI：
10.1046/j.1439-0523.2001.00636.x
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发表时间：
2001-10-01
期刊：
影响因子：
2
通讯作者：
Quiros, CF
中科院分区：
文献类型：
--
作者：
Riaz, A;Li, G;Quiros, CF
油菜种子产量的显著杂种优势引起了人们对杂交油菜品种开发的兴趣。采用基于DNA的分子标记技术--序列相关扩增多态性（SRAP）技术对油菜保持系和恢复系的遗传多样性进行了研究。利用这一方法试图建立遗传距离与杂种优势之间的关系。利用IS SRAP引物组合共扩增出118个多态位点。基于标记产生的多态性，计算的相似性指数值范围为0.46至0.97。聚类分析将10个保持系和12个恢复系分为3类，但保持系PM 5和PM 9不在这3类中。品系的分组在很大程度上与关于其起源和农艺性能的可用系谱数据一致。在两个地点的自交系和它们产生的F，杂交种之间的方差分析显示株高，成熟天数和种子产量的显着差异，但不含油量。仅在种子产量上观察到显著的中亲优势，其范围为26%至169%。所有的杂交种都超过了各自的自交系的这一特性，除了一个单一的交叉组合的相关线。一般来说，位于不同聚类中的品系的杂交比来自相同聚类的品系的杂交产生更多的产量。回归分析表明，亲本间的遗传距离与杂交种的种子产量、中亲优势和高亲优势之间存在显著的相关关系。遗传距离与产量的相关系数为0.64，表明部分SRAP标记可能与种子产量的数量性状位点连锁。
Significant heterosis for seed yield in oilseed rape has created interest in the development of hybrid cultivars. The DNA-based marker protocol, sequence-related amplified polymorphism (SRAP) was used to determine genetic diversity among oilseed rape maintainer and restorer lines. This measure was used in an attempt to establish an association between genetic distance and heterosis in hybrids for various agronomic traits. A total of I 18 polymorphic loci were generated by IS SRAP primer combinations. Based on the polymorphisin generated by the markers, calculated similarity index values ranged from 0.46 to 0.97. Cluster analysis grouped 10 maintainer and 12 restorer lines into three groups, with the exception of two maintainer lines, PM5 and PM9, which fell outside these groups. The grouping of the lines was largely in agreement with the available pedigree data on their origin and agronomic performance. Analysis of variance among inbred lines and their resulting F, hybrids over two locations revealed significant differences for plant height, days to maturity and seed yield, but not for oil content. Substantial mid-parent heterosis was observed only for seed yield, and ranged from 26% to 169%. All hybrids surpassed their respective inbred lines for this trait, except for a single cross combination of related lines. In general, crosses of lines located in different clusters yielded more than those from the same clusters. Regression analysis revealed a statistically significant relationship between the genetic distance of the parents and seed yield in their hybrid, and their derived mid-parent and high-parent heterosis. The correlation coefficient between genetic distance and yield (0.64) indicated a moderately strong relationship, so it is possible that some of the SRAP markers might be linked to quantitative trait loci for seed yield.