Expression profile of embryonic stem cell-associated genes Oct4, Sox2 and Nanog in human gliomas
Expression profile of embryonic stem cell-associated genes Oct4, Sox2 and Nanog in human gliomas
复制标题
DOI：
10.1111/j.1365-2559.2011.03993.x
复制
发表时间：
2011-10-01
期刊：
影响因子：
6.4
通讯作者：
Hao, Aijun
中科院分区：
文献类型：
--
作者：
Guo, Yuji;Liu, Shangming;Hao, Aijun
目的：目的探讨维持胚胎干细胞（embryonic stem cells，ESCs）多能性和自我更新的3个核心调控因子Oct4、Sox 2和Nanog在人脑胶质瘤中是否共表达，以及它们的表达是否与肿瘤的发生和肿瘤干细胞（cancer stem cells，CSCs）的形成有关。采用免疫组化、逆转录聚合酶链反应和western blot检测Oct4、Sox 2和Nanog的表达。我们发现Oct4、Sox2和Nanog的表达水平与肿瘤恶性程度呈正相关。免疫组化显示Oct4和Nanog在胶质瘤细胞的胞核和胞浆中均有表达，而Sox 2仅在细胞核中表达。免疫荧光双重染色显示，大多数Oct4阳性细胞共表达Sox 2和Nanog。超过50%的Oct4阳性细胞共表达推定的CSC标志物CD133和Nestin。此外，一些细胞表现出Oct4和Nanog的免疫表达在细胞质中，但阳性细胞的频率并不与肿瘤malignancy.Conclusions：目前的研究结果表明，ESC相关的通路被激活在人脑胶质瘤，这些可能参与胶质瘤的进展，一个作用，是不同的，在ESC。
Aims: To investigate whether Oct4, Sox2 and Nanog, three core regulatory factors maintaining pluripotency and self-renewal of embryonic stem cells (ESCs), are coexpressed in human gliomas, and whether their expression might be linked to carcinogenesis and the formation of cancer stem cells (CSCs).Methods and results: Forty cases of human glioma were examined. The expression of Oct4, Sox2 and Nanog was analysed by immunohistochemistry, reverse transcription polymerase chain reaction and western blot. We found a positive correlation between the expression levels of Oct4, Sox2 and Nanog and tumour malignancy. Immunohistochemistry showed that Oct4 and Nanog were expressed in both the nuclei and the cytoplasm of glioma cells, whereas Sox2 was expressed only in the nuclei. Double immunofluorescence staining revealed that a majority of Oct4-positive cells coexpressed Sox2 and Nanog. More than 50% of Oct4-positive cells coexpressed the putative CSC markers CD133 and Nestin. Moreover, some cells exhibited Oct4 and Nanog immunoexpression in the cytoplasm, but the frequency of positive cells did not correlate with tumour malignancy.Conclusions: The present findings suggest that ESC-associated pathways are activated in human gliomas and that these may be involved in glioma progression, a role that is distinct from that in ESCs.