Characterization, antioxidant activity and hepatoprotective effect of purple sweetpotato polysaccharides
Characterization, antioxidant activity and hepatoprotective effect of purple sweetpotato polysaccharides
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紫甘薯多糖的表征、抗氧化活性及保肝作用
DOI：
10.1016/j.ijbiomac.2018.04.033
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发表时间：
2018
影响因子：
8.2
通讯作者：
Nianfeng Zhang
中科院分区：
文献类型：
--
作者：
Jian Sun;Bo Zhou;Chao Tang;Yarun Gou;Hong Chen;Yao Wang;Changhai Jin;Jun Liu;Fuxiang Niu;Juan Kan;Chunlu Qian;Nianfeng Zhang
本研究采用热水、0.5M和2M氢氧化钠溶液从紫甘薯块茎中连续分离得到3种多糖，分别命名为PSWP、PSAP-1和PSAP-2。对它们的性质、体外抗氧化活性和体内保肝作用进行了研究。结果表明，PSWP、PSAP-1和PSAP-2均为糖醛酸、蛋白质和多酚含量不同的β类多糖。PSWP和PSAP-1均由阿拉伯糖、葡萄糖和半乳糖组成，而PSAP-2由阿拉伯糖、鼠李糖和葡萄糖组成。所有的多糖都具有中等的2，2-二苯基-1-苦基肼(DPPH)清除活性和还原能力。与四氯甲烷(CCl4)组相比，PSWP、PSAP-1和PSAP-2组小鼠血清碱性磷酸酶、丙氨酸转氨酶和天冬氨酸转氨酶水平降低，肝脏脂质过氧化水平降低，而肝脏抗氧化酶(超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶)和非酶类抗氧化剂(谷胱甘肽)水平升高。值得注意的是，PSWP表现出比PSAP-1和PSAP-2更强的抗氧化活性和保肝作用。PSWP的保肝作用与水飞蓟素阳性标准相当。结果表明，紫甘薯多糖具有潜在的抗氧化活性和对CCl4所致急性肝损伤的保护作用。
In this study, three kinds of polysaccharides (named PSWP, PSAP-1 and PSAP-2) were successively isolated from purple sweetpotato tubers by hot water, 0.5 M and 2 M sodium hydroxide solutions. The characterization,in vitroantioxidant activity andin vivohepatoprotective effect of these polysaccharides were investigated. Results indicated that PSWP, PSAP-1 and PSAP-2 were all β-type polysaccharides containing different contents of uronic acid, protein and polyphenol. Both PSWP and PSAP-1 were composed by arabinose, glucose and galactose, whereas PSAP-2 was consisted of arabinose, rhamnose and glucose. All the polysaccharides exhibited moderate 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity and reducing power. As compared with tetrachloromethane (CCl4) treatment group, mice administrated with PSWP, PSAP-1 and PSAP-2 exhibited decreased levels of serum enzymes (alkaline phosphatase, alanine transaminase and aspartate transaminase) and hepatic lipid peroxidation, whereas increased levels of hepatic antioxidant enzymes (superoxide dismutase, catalase and glutathione peroxidase) and non-enzymatic antioxidant (glutathione). Notably, PSWP displayed stronger antioxidant activity and hepatoprotective effect than PSAP-1 and PSAP-2. The hepatoprotective effect of PSWP was comparable to positive standard of silymarin. Our results suggested polysaccharides from purple sweetpotato possessed potential antioxidant activity and protective effect against CCl4-induced acute liver damage.