Cerebral Hypoxia—Ischemia in Neonatal Rats or Mice: A Model of Perinatal Brain Injury
Cerebral Hypoxia—Ischemia in Neonatal Rats or Mice: A Model of Perinatal Brain Injury
复制标题
脑缺氧——新生大鼠或小鼠缺血：围产期脑损伤模型
DOI：
10.1007/978-1-60327-185-1_18
复制
发表时间：
2009
期刊：
影响因子：
2.9
通讯作者：
K. Blomgren
中科院分区：
文献类型：
--
作者：
Changlian Zhu;Xiaoyang Wang;K. Blomgren;K. Blomgren
围产期脑损伤的最广泛的动物模型是新生啮齿动物脑缺氧-缺血（HI）模型。缺血性脑损伤是通过单侧颈总动脉结扎后中度缺氧产生的。单独的颈动脉结扎不会引起任何损伤，单独的缺氧也不会引起任何损伤，但联合使用会在结扎同侧的大脑半球的皮质、海马、纹状体和丘脑中产生多灶性缺血性损伤，而对侧大脑半球未受损伤。缺氧时同侧大脑半球的血流量减少，恢复常氧时血流量恢复正常。因此，这是一个脑缺血和再灌注模型，尽管永久性单侧颈动脉结扎。该模型具有良好的特性，易于实施，并允许长期生存。低成本允许纳入足够数量的动物来研究围产期HI脑损伤的生理和分子机制，以及测试潜在的保护性治疗和评估长期功能结果。缺点是不同动物的损伤程度可能不同，但这可以通过使用更多数量的动物来补偿。最初是为大鼠开发的，它也被应用于新生小鼠，使转基因品系的使用成为可能。该模型可用于不同的发育阶段，允许比较例如早产儿、足月儿、青少年或成人脑损伤的等同物。
The most widespread animal model of perinatal brain injury is the neonatal rodent cerebral hypoxia–ischemia (HI) model. Ischemic brain injury is produced through unilateral common carotid artery ligation followed by moderate hypoxia. Carotid ligation alone does not induce any injury, nor does hypoxia alone, but the combination produces multifocal ischemic injury in the cortex, hippocampus, striatum, and thalamus in the hemisphere ipsilateral to the ligation, leaving the contralateral hemisphere uninjured. Blood flow is reduced in the ipsilateral hemisphere during hypoxia and returns to normal levels when normoxia is restored. Hence, this is a model of cerebral ischemia and reperfusion, despite permanent unilateral carotid artery ligation. The model is well characterized, easy to carry out, and allows long-term survival. The low cost allows inclusion of sufficient numbers of animals to study physiological and molecular mechanisms of perinatal HI brain injury, as well as testing potentially protective treatments and evaluating long-term functional outcome. A disadvantage is that the extent of injury may vary between animals, but this can be compensated for by using higher numbers of animals. Originally developed for rats, it has also been applied to the neonatal mouse, enabling use of genetically modified strains. The model can be used at different developmental stages, allowing comparison of, for example, the equivalent of preterm, term, juvenile, or adult brain injury.
DOI：
10.1016/s1071-9091(99)80046-1
发表时间：
1999
期刊：
Seminars in pediatric neurology.
影响因子：
--
作者：
Vannucci,RC;Towfighi,J
通讯作者：
Towfighi,J