A prokaryotic dnaA sequence in Drosophila melanogaster: Wolbachia infection and cytoplasmic incompatibility among laboratory strains
A prokaryotic dnaA sequence in Drosophila melanogaster: Wolbachia infection and cytoplasmic incompatibility among laboratory strains
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DOI：
10.1111/j.1365-2583.1994.tb00160.x
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发表时间：
1994-01-01
影响因子：
2.6
通讯作者：
Savakis, C.
中科院分区：
文献类型：
--
作者：
Bourtzis, K.;Nirgianaki, A.;Savakis, C.
使用源自几个原核 dnaA 基因的比对多肽序列的寡核苷酸引物，我们从果蝇 DNA 中扩增出包含单个开放阅读框的 557 bp 片段。预测的肽序列与先前表征的由几种原核生物的 dnaA 基因编码的蛋白质序列显着相似。由几种原核生物的 dnaA 基因编码的 dnaA 序列。 dnaA 序列也可以通过 PCR 在来自模拟果蝇和 Nasonia vitripennis 果蝇的 DNA 中检测到，这些果蝇被属于模式种 Wolbachia pipientis 的共生细菌感染。四环素治疗可以根除昆虫中的细菌寄生虫，消除果蝇和 Nasonia DNA 中的 dnaA 序列。此外，dnaA阳性的黑腹果蝇胚胎中含有大量杆状细菌，这些细菌在经过四环素处理后在后代中被消除。结合DnaA和16S rRNA序列的系统发育分析，这些结果表明dnaA同源来自Wolbachia。使用 dnaA 和 16S rRNA 序列的 PCR 扩增对果蝇种群进行的调查表明，沃尔巴克氏体在黑腹果蝇实验室菌株中广泛传播，但在地中海果蝇 Ceratitis headata 的几个已建立菌株中不存在。还有证据表明，该细菌的存在会导致受感染和未受感染的黑腹果蝇菌株之间部分细胞质不相容。
Using oligonucleotide primers derived from the aligned polypeptide sequences of several prokaryotic dnaA genes, we amplified from Drosophila melanogaster DNA a 557 bp fragment containing a single open reading frame. The predicted peptide sequence shows a significant similarity to previously characterized protein sequences that are encoded by the dnaA genes of several prokaryotes. The dnaA sequences that are encoded by the dnaA genes of several prokaryotes. The dnaA sequences are also detectable by PCR in DNA from Drosophila simulans and Nasonia vitripennis flies which are infected by a symbiotic bacterium assigned to the type species Wolbachia pipientis. A tetracycline treatment that eradicates bacterial parasites from insects, abolishes the dnaA sequences from Drosophila and Nasonia DNA. In addition, dnaA-positive Drosophila melanogaster contain numerous rod-shaped bacteria in embryos, which are abolished in subsequent generations after treatment with tetracycline. Combined with phylogenetic analysis of DnaA and 16S rRNA sequences, these results show that the dnaA cognate comes from Wolbachia. A survey of Drosophila stocks using PCR amplification of dnaA and 16S rRNA sequences showed that Wolbachia is widely spread among D. melanogaster laboratory strains but absent from several established strains of the Mediterranean fruit fly Ceratitis capitata. Evidence is also presented that presence of the bacterium can cause partial cytoplasmic incompatibility between infected and noninfected D. melanogaster strains.