A single-cell atlas of Drosophila trachea reveals glycosylation-mediated Notch signaling in cell fate specification
A single-cell atlas of Drosophila trachea reveals glycosylation-mediated Notch signaling in cell fate specification
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DOI：
10.1038/s41467-024-46455-w
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发表时间：
2024-03-06
影响因子：
16.6
通讯作者：
Huang，Hai
中科院分区：
文献类型：
--
作者：
Li，Yue;Lu，Tianfeng;Huang，Hai
果蝇气管系统是研究管状形态发生程序的有利模型。该系统是由有丝分裂后细胞在胚胎中建立的，但也经历成体干细胞的重塑。在这里，我们使用单细胞 RNA 测序 (scRNA-seq) 技术提供了果蝇气管的综合细胞图谱。该图谱记录了果蝇气道内气管母细胞的转录谱，描绘了 9 个主要亚型。从计算机和基因研究中获得的进一步证据强调了一组转录因子，其特征在于它们具有改变细胞命运的能力。值得注意的是，转录因子 Pebbled、Blistered、Knirps、Spalt 和 Cut 受 Notch 信号传导影响并决定气管细胞身份。此外，Notch 信号传导协调气管细胞分化所必需的转录活动，并对高糖饮食诱导的蛋白质糖基化做出反应。因此，我们的研究产生了气管发育和再生的单细胞转录组图谱，并提出了细胞命运决定中糖基化响应的Notch信号传导。
TheDrosophilatracheal system is a favorable model for investigating the program of tubular morphogenesis. This system is established in the embryo by post-mitotic cells, but also undergoes remodeling by adult stem cells. Here, we provide a comprehensive cell atlas ofDrosophilatrachea using the single-cell RNA-sequencing (scRNA-seq) technique. The atlas documents transcriptional profiles of tracheoblasts within theDrosophilaairway, delineating 9 major subtypes. Further evidence gained from in silico as well as genetic investigations highlight a set of transcription factors characterized by their capacity to switch cell fate. Notably, the transcription factors Pebbled, Blistered, Knirps, Spalt and Cut are influenced by Notch signaling and determine tracheal cell identity. Moreover, Notch signaling orchestrates transcriptional activities essential for tracheoblast differentiation and responds to protein glycosylation that is induced by high sugar diet. Therefore, our study yields a single-cell transcriptomic atlas of tracheal development and regeneration, and suggests a glycosylation-responsive Notch signaling in cell fate determination.