Characterization of a Feruloyl Esterase from Lactobacillus plantarum
Characterization of a Feruloyl Esterase from Lactobacillus plantarum
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DOI：
10.1128/aem.01523-13
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发表时间：
2013-09-01
影响因子：
4.4
通讯作者：
Munoz, Rosario
中科院分区：
文献类型：
--
作者：
Esteban-Torres, Maria;Reveron, Ines;Munoz, Rosario
植物乳杆菌经常在富含羟基肉桂酸酯的植物源性食品的发酵中发现。L. plantarumWCFS 1培养物不能水解羟基肉桂酰酯;然而，来自该菌株的细胞提取物部分水解阿魏酸甲酯和对香豆酸甲酯。为了发现蛋白质Lp_0796是否是负责该水解活性的酶，将其重组过量生产并进行酶促表征。Lp_0796是一种酯酶，除了其他底物外，其能够有效水解阿魏酸酯酶的四种模型底物（阿魏酸甲酯、咖啡酸甲酯、对香豆酸甲酯和芥子酸甲酯）。对编码阿魏酸酯酶Lp_0796基因的筛选试验表明，该基因普遍存在于L.植物菌株本文报道了L. plantarum，并提供了新的见解羟基肉桂化合物在这种细菌物种的代谢。
Lactobacillus plantarum is frequently found in the fermentation of plant-derived food products, where hydroxycinnamoyl esters are abundant. L. plantarum WCFS1 cultures were unable to hydrolyze hydroxycinnamoyl esters; however, cell extracts from the strain partially hydrolyze methyl ferulate and methyl p-coumarate. In order to discover whether the protein Lp_0796 is the enzyme responsible for this hydrolytic activity, it was recombinantly overproduced and enzymatically characterized. Lp_0796 is an esterase that, among other substrates, is able to efficiently hydrolyze the four model substrates for feruloyl esterases (methyl ferulate, methyl caffeate, methyl p-coumarate, and methyl sinapinate). A screening test for the detection of the gene encoding feruloyl esterase Lp_0796 revealed that it is generally present among L. plantarum strains. The present study constitutes the description of feruloyl esterase activity in L. plantarum and provides new insights into the metabolism of hydroxycinnamic compounds in this bacterial species.