Multiphoton imaging of renal regulatory mechanisms.
Multiphoton imaging of renal regulatory mechanisms.
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DOI：
10.1152/physiol.00001.2009
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发表时间：
2009-04
期刊：
影响因子：
--
通讯作者：
Vargas SL
中科院分区：
文献类型：
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作者：
Peti-Peterdi J;Toma I;Sipos A;Vargas SL
肾脏的大多数生理功能，包括代谢废物的清除、体液的维持、电解质稳态和血压，是通过多种肾细胞类型与许多器官部分中先前难以接近的结构之间的复杂相互作用来实现的，这些结构一直难以研究。多光子荧光显微镜提供了一种先进的成像技术，用于活体组织和器官的深度光学切片，并具有最小的有害影响。完整肾脏中的动态调节过程和多种功能可以在真实的时间、非侵入性和亚微米分辨率下定量可视化。本文综述了创新的多光子成像技术及其应用，这些技术提供了最复杂、最直接和最动态的肾功能描述，清晰地描绘并分析了肾（病理）生理学中涉及的成分和机制。
Most physiological functions of the kidneys, including the clearance of metabolic waste products, maintenance of body fluid, electrolyte homeostasis, and blood pressure, are achieved by complex interactions between multiple renal cell types and previously inaccessible structures in many organ parts that have been difficult to study. Multiphoton fluorescence microscopy offers a state-of-the-art imaging technique for deep optical sectioning of living tissues and organs with minimal deleterious effects. Dynamic regulatory processes and multiple functions in the intact kidney can be quantitatively visualized in real time, noninvasively, and with submicron resolution. This article reviews innovative multiphoton imaging technologies and their applications that provided the most complex, immediate, and dynamic portrayal of renal function—clearly depicting as well as analyzing the components and mechanisms involved in renal (patho)physiology.