Fragment detection of Coleopteran and Triatomine insects in experimentally contaminated acai pulp and sugarcane juice
Fragment detection of Coleopteran and Triatomine insects in experimentally contaminated acai pulp and sugarcane juice
复制标题
实验污染的巴西莓果肉和甘蔗汁中鞘翅目和锥蝽昆虫的碎片检测
DOI：
10.1590/0037-8682-0119-2019
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发表时间：
2019
影响因子：
2
通讯作者：
V. Pereira‐Chioccola
中科院分区：
文献类型：
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作者：
E. C. Mattos;M. A. M. Marciano;V. D. S. M. Daros;C. Faccini;Angela Maria Lourenço;V. Pereira‐Chioccola
摘要前言：急性恰加斯病的口腔传播是一个新兴的公共卫生问题。这项研究的目的是通过将三聚氰胺与其他昆虫进行比较，检测被实验污染的食物中的昆虫碎片。方法：对食品样品进行昆虫污染实验，轻污处理回收碎片，用显微镜和聚合酶链式反应(PCR)进行分析。结果：在显微图像上观察到鞘翅目昆虫和三甲虫昆虫的形态差异。聚合酶链式反应能有效地扩增实验污染食品中的螺旋藻DNA。结论：食品分析人员可以利用这一方法来确定食品样品中可能存在的昆虫污染。
Abstract INTRODUCTION: Oral transmission of acute Chagas disease is an emerging public health concern. This study aimed to detect insect fragments in experimentally contaminated food, by comparing triatomines with other insects. METHODS: Food samples were experimentally contaminated with insects, processed to recover their fragments by light filth, and analyzed by microscopy and Polymerase Chain Reaction (PCR). RESULTS: Morphological differences between coleopteran and triatomine insects were observed in microscopic images. PCR was efficient in amplifying Triatominae DNA in the experimentally contaminated food. CONCLUSIONS: This methodology could be utilized by food analysts to identify possible insect contamination in food samples.