The automated bioaerosol exposure system: Preclinical platform development and a respiratory dosimetry application with nonhuman primates
The automated bioaerosol exposure system: Preclinical platform development and a respiratory dosimetry application with nonhuman primates
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DOI：
10.1016/j.vascn.2003.07.001
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发表时间：
2004-01-01
影响因子：
1.9
通讯作者：
Roy, Chad J.
中科院分区：
文献类型：
--
作者：
Hartings, Justin M.;Roy, Chad J.
简介：充分表征的吸入暴露系统对于临床前试验和发病机制研究至关重要。自动化生物气溶胶暴露系统（ABES）提供了一个微处理器驱动的吸入平台，可对吸入暴露的各个方面进行精密的数据采集和控制。由于这是一项新技术，因此详细讨论了ABES的发展和特点。除了控制体内平衡和气溶胶条件外，ABES还结合了基于吸入期间试验动物呼吸性能的剂量测定功能。方法：为了测试该系统，恒河猴最初使用ABES以仅头部吸入配置进行假暴露。随后在生物安全水平（BSL）-III条件下，使用雾化葡萄球菌肠毒素B（SE B）毒素进行疫苗效力挑战，再次使用系统的实时剂量测定功能。结果如下：假暴露结果表明与吸入程序前进行的相应全身体积描记（WBP）呼吸功能估计值存在显著偏离。SEB暴露的结果证明了使用ABES产生一致准确和精确的吸入剂量的效用。讨论内容：总之，假手术和毒素挑战实验的结果表明，与暴露前进行的预测WBP相比，ABES的剂量测定功能提高了吸入剂量输送和计算的精度和准确度。ABES代表了一个高度适应性的平台，吸入系统的设计，以适应各种动物models.Introduction的要求：良好的特点吸入暴露系统的临床前测试和发病机制研究是至关重要的。自动化生物气溶胶暴露系统（ABES）提供了一个微处理器驱动的吸入平台，可对吸入暴露的各个方面进行精密的数据采集和控制。由于这是一项新技术，因此详细讨论了ABES的发展和特点。除了控制体内平衡和气溶胶条件外，ABES还结合了基于吸入期间试验动物呼吸性能的剂量测定功能。方法：为了测试该系统，恒河猴最初使用ABES以仅头部吸入配置进行假暴露。随后在生物安全水平（BSL）-III条件下，使用雾化葡萄球菌肠毒素B（SE B）毒素进行疫苗效力挑战，再次使用系统的实时剂量测定功能。结果如下：假暴露结果表明与吸入程序前进行的相应全身体积描记（WBP）呼吸功能估计值存在显著偏离。SEB暴露的结果证明了使用ABES产生一致准确和精确的吸入剂量的效用。讨论内容：总之，假手术和毒素挑战实验的结果表明，与暴露前进行的预测WBP相比，ABES的剂量测定功能提高了吸入剂量输送和计算的精度和准确度。ABES代表了一个高度适应性的吸入系统设计平台，以满足各种动物模型的要求。(C)2003年爱思唯尔公司All rights reserved.
Introduction: Well-characterized inhalation exposure systems are critical for preclinical testing and pathogenesis studies. The automated bioaerosol exposure system (ABES) provides a microprocessor-driven inhalation platform that provides exquisite data acquisition and control over all aspects of inhalation exposures. Because this represents a new technology, the development and characteristics of the ABES are thoroughly discussed. In addition to control over homeostatic and aerosol conditions, the ABES incorporates a dosimetry function based on respiratory performance of the test animal during inhalation. Methods: To test the system, rhesus macaques were initially sham-exposed using the ABES in a head-only inhalation configuration. The ABES was subsequently used under biosafety level (BSL)-III conditions in a vaccine efficacy challenge using aerosolized staphylococcal enterotoxin B (SEB) toxin, again using the real-time dosimetry function of the system. Results: Sham exposure results indicated significant departures from corresponding whole-body plethysmography (WBP) respiratory function estimates taken before the inhalation procedure. The results of the SEB exposure demonstrated the utility of using the ABES to generate consistently accurate and precise inhalation dose. Discussion: Taken together, the results of the sham and toxin challenge experiments demonstrate that the dosimetry function of the ABES improves the precision and accuracy of inhaled dose delivery and calculation as compared to predictive WBP conducted before the exposure. The ABES represents a highly adaptable platform for the design of inhalation systems to suit the requirements of a variety of animal models.Introduction: Well-characterized inhalation exposure systems are critical for preclinical testing and pathogenesis studies. The automated bioaerosol exposure system (ABES) provides a microprocessor-driven inhalation platform that provides exquisite data acquisition and control over all aspects of inhalation exposures. Because this represents a new technology, the development and characteristics of the ABES are thoroughly discussed. In addition to control over homeostatic and aerosol conditions, the ABES incorporates a dosimetry function based on respiratory performance of the test animal during inhalation. Methods: To test the system, rhesus macaques were initially sham-exposed using the ABES in a head-only inhalation configuration. The ABES was subsequently used under biosafety level (BSL)-III conditions in a vaccine efficacy challenge using aerosolized staphylococcal enterotoxin B (SEB) toxin, again using the real-time dosimetry function of the system. Results: Sham exposure results indicated significant departures from corresponding whole-body plethysmography (WBP) respiratory function estimates taken before the inhalation procedure. The results of the SEB exposure demonstrated the utility of using the ABES to generate consistently accurate and precise inhalation dose. Discussion: Taken together, the results of the sham and toxin challenge experiments demonstrate that the dosimetry function of the ABES improves the precision and accuracy of inhaled dose delivery and calculation as compared to predictive WBP conducted before the exposure. The ABES represents a highly adaptable platform for the design of inhalation systems to suit the requirements of a variety of animal models. (C) 2003 Elsevier Inc. All rights reserved.