Chromosomal location of an uncharacterised stripe rust resistance gene in wheat
Chromosomal location of an uncharacterised stripe rust resistance gene in wheat
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DOI：
10.1007/s10681-009-0007-4
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发表时间：
2010-01-01
期刊：
影响因子：
1.9
通讯作者：
Bariana, H. S.
中科院分区：
文献类型：
--
作者：
Bansal, U. K.;Hayden, M. J.;Bariana, H. S.
最近在西澳大利亚引进的小麦条锈病病型134 E16A+对条锈病抗性基因Yr8和Yr9的毒力不同，对Yr3、Yr4、Yr34和一些未鉴定的抗性来源的毒力不同。澳大利亚小麦品种Rubric对Pst病型134 E16A+表现出极低的幼苗条锈病反应。对Rubric/Avocet 'S' F-3群体的遗传分析表明，抗性具有单基因遗传。抗性基因暂定为YrRub。利用多重聚合PCR技术进行批量分离分析，将YrRub置于3BS染色体微卫星标记barc75的远端。后续研究在YrRub （2.9 +/- A 1.3 cM）和barc75 （2.4 +/- A 1.2 cM）之间定位了SSR标记cfb3530。Bolac、EMU’S、Nesser、Hybrid 46 （Yr4）和Avalon （Yr4）基因型扩增了与yrrubb连锁的barc75和cfb3530等位基因。在携带Yr4的品种“杂交46”和“阿瓦隆”中扩增出与Rubric相似的PCR产物，表明YrRub极有可能是Yr4。由于缺乏单独携带Yr4的基因型，无法进行等位基因检测。在38个已知缺乏YrRub的澳大利亚小麦品种中，WAWHT2046 （Yr34）和Vilmorin 23 （Yr3）在barc75和cfb3530标记位点上扩增出的PCR产物与Rubric不同。在不同的小麦基因型中，barc75和cfb3530位点的非rubric等位基因扩增表明，这些标记可与其他分子标记的小麦幼苗和成株抗条锈病基因联合用于小麦育种中的标记辅助选择。
A recent introduction of Puccinia striiformis f. sp. tritici (Pst) pathotype 134 E16A+ in Western Australia differed from existing pathotypes in its virulence for stripe rust resistance genes Yr8 and Yr9, and avirulence for Yr3, Yr4, Yr34 and some uncharacterized sources of resistance. The Australian wheat cultivar Rubric exhibited a very low seedling stripe rust response when tested against the Pst pathotype 134 E16A+. Genetic analysis of a Rubric/Avocet 'S' F-3 population indicated monogenic inheritance of resistance. The resistance gene was tentatively designated YrRub. Bulked segregant analysis using multiplex-ready PCR technology placed YrRub distal to the microsatellite marker barc75 in chromosome 3BS. Follow up studies mapped SSR marker cfb3530 between YrRub (2.9 +/- A 1.3 cM) and barc75 (2.4 +/- A 1.2 cM). Genotypes Bolac, EMU 'S', Nesser, Hybrid 46 (Yr4) and Avalon (Yr4) amplified the YrRub-linked barc75 and cfb3530 alleles. The amplification of PCR products similar to that of Rubric in Yr4-carrying cultivars Hybrid 46 and Avalon suggested that YrRub was most likely to be Yr4. Due to unavailability of genotypes carrying Yr4 singly, test of allelism was not possible. Thirty-eight Australian wheat cultivars known to lack YrRub, an advanced breeding line WAWHT2046 (Yr34) and Vilmorin 23 (Yr3) amplified PCR products different from that of Rubric at the barc75 and cfb3530 marker loci. The amplification of non-Rubric alleles at the marker barc75 and cfb3530 loci among a set of diverse wheat genotypes demonstrated that these markers could be used for marker assisted selection of YrRub in combination with other molecularly tagged seedling and adult plant stripe rust resistance genes in wheat breeding programs.