2-[(4-Azido-2-nitrophenyl)amino]ethyl triphosphate, a novel chromophoric and photoaffinity analogue of ATP. Synthesis, characterization, and interaction with myosin subfragment 1.
2-[(4-Azido-2-nitrophenyl)amino]ethyl triphosphate, a novel chromophoric and photoaffinity analogue of ATP. Synthesis, characterization, and interaction with myosin subfragment 1.
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2-[(4-叠氮基-2-硝基苯基)氨基]乙基三磷酸，一种新型发色和光亲和的 ATP 类似物。
DOI：
10.1021/bi00340a041
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发表时间：
1985
期刊：
影响因子：
2.9
通讯作者：
Yount,RG
中科院分区：
文献类型：
--
作者：
Nakamaye,KL;Wells,JA;Bridenbaugh,RL;Okamoto,Y;Yount,RG
材料与方法ATP和ADP的钠盐来自PL Biochemicals。[2，8- 3 H] AMP-PNP和[32 P]正磷酸钠来自ICN。如Guillory和Jeng（1977）所述制备4-氟-3-硝基苯基叠氮化物，并且与购自Pierce Chemical Co.1的相同化合物相同。1，1 ′-羰基二咪唑和六甲基磷酰胺购自Aldrich。pPDM、oPDM和1，10-菲咯啉来自Aldrich; F2 DPS和DTNB来自Pierce。[Com-（phen）2CO 3] Cl根据Ablov和Palade（1961）如前所述制备（威尔斯等人，1979年）。三乙基碳酸氢铵溶液的制备、非放射性AMP-PNP的合成以及总磷酸盐和酸不稳定磷酸盐的分析如所述（Yount等人，1971年）。（Na++ K+）-ATP酶制剂来自Sigma。F-ATP酶是哈维·佩内夫斯基博士慷慨的礼物。
Materials and MethodsSodium salts of ATP and ADP were from PL Biochemicals.[2, 8-3H] AMP-PNP and sodium [32P] orthophosphate were from ICN. 4-Fluoro-3-nitrophenyl azide was prepared as described by Guillory & Jeng (1977) and was identical with the same compound purchased from the Pierce Chemical Co.1, 1'-Carbonyldiimidazole and hexamethylphosphoramide were from Aldrich. pPDM, oPDM, and 1, 10-phenanthroline were from Aldrich; F2DPS and DTNB were from Pierce.[Com-(phen) 2C03] Cl was prepared according to Ablov & Palade (1961) as previously described (Wells et al., 1979). Prepa-ration of triethylammonium bicarbonate solutions, synthesis of nonradioactive AMP-PNP, and analyses fortotal phosphate and acid-labile phosphate were as described (Yount et al., 1971). The (Na++ K+)-ATPase preparations were from Sigma. F,-ATPase was a generous gift of Dr. Harvey Pe-nefsky.