PNMT-containing neurons of the C1 cell group express c-fos in response to changes in baroreceptor input.
PNMT-containing neurons of the C1 cell group express c-fos in response to changes in baroreceptor input.
复制标题
C1 细胞群中含有 PNMT 的神经元表达 c-fos，以响应压力感受器输入的变化。
DOI：
10.1152/ajpregu.1994.266.2.r361
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Hoffman,GE
中科院分区：
文献类型：
--
作者：
Sved,AF;Mancini,DL;Graham,JC;Schreihofer,AM;Hoffman,GE
Fos（立即早期基因 c-fos 的蛋白质产物）的免疫细胞化学检测被用作激活神经元的标记，以检查大鼠头端腹外侧延髓 (RVLM) 中的 C1 神经元是否对压力感受器传入活动的变化做出反应。在肼屈嗪诱导的低血压或去主动脉神经支配后，两种减少压力感受器传入活性的治疗方法，在 RVLM 中观察到大量 Fos 阳性神经元。计算 RVLM 中 Fos 阳性神经元的数量，这些大鼠的脑干切片来自肼苯哒嗪治疗的大鼠，这些大鼠之前已将 Fluorogold 注射到上胸脊髓中，以标记脊髓突出的 RVLM 神经元，并进行苯乙醇胺-N-甲基转移酶 (PNMT) 染色作为 C1 神经元的标记。结果表明，大约 80% 的 C1 神经元响应肼苯达嗪注射而表达 Fos；对于脊髓投射的 C1 神经元以及那些未用 Fluorogold 标记的 C1 神经元来说，情况都是如此。此外，在肼苯哒嗪治疗的大鼠中，大多数 Fluogold 标记的 Fos 阳性神经元含有 PNMT。这些结果表明 C1 神经元对压力感受器传入输入敏感，并支持这些神经元在心血管调节中的作用。
The immunocytochemical detection of Fos, the protein product of the immediate-early gene c-fos, was used as a marker for activated neurons to examine whether the C1 neurons in the rat rostral ventrolateral medulla (RVLM) respond to changes in baroreceptor afferent activity. After hydralazine-induced hypotension or sinoaortic denervation, two treatments that reduce baroreceptor afferent activity, numerous Fos-positive neurons were observed in the RVLM. The number of Fos-positive neurons in the RVLM was counted in brain stem sections from hydralazine-treated rats that had been previously injected with Fluorogold into the upper thoracic spinal cord to label spinally projecting RVLM neurons as well as stained for phenylethanolamine-N-methyltransferase (PNMT) as a marker of C1 neurons. The results indicate that approximately 80% of the C1 neurons expressed Fos in response to hydralazine injection; this was true of spinally projecting C1 neurons as well as those C1 neurons that were not labeled with Fluorogold. Furthermore, in hydralazine-treated rats, the majority of Fluorogold-labeled Fos-positive neurons contained PNMT. These results suggest that C1 neurons are sensitive to baroreceptor afferent input and support a role of these neurons in cardiovascular regulation.