Molecular analysis of the key cytokinetic components of cyanobacteria: FtsZ, ZipN and MinCDE
Molecular analysis of the key cytokinetic components of cyanobacteria: FtsZ, ZipN and MinCDE
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DOI：
10.1111/j.1365-2958.2004.04042.x
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发表时间：
2004-05-01
影响因子：
3.6
通讯作者：
Chauvat, F
中科院分区：
文献类型：
--
作者：
Mazouni, K;Domain, F;Chauvat, F
利用细菌双杂交系统和结合的体内和体外试验，利用绿色荧光报告蛋白（GFP），我们已经调查了定位和蛋白质-蛋白质相互作用的几个关键组成部分的细胞动力学机制的蓝藻（即叶绿体的祖先）。我们证明了（i）ftsZ和zipN基因对模式蓝藻集胞藻PCC 6803的生存能力是必需的，而minCDE簇对细胞生长是必需的;（ii）FtsZ的GTP结合结构域对FtsZ组装到细胞中间的隔环中是至关重要的;（iii）深度收缩的子细胞的Z环垂直于母Z环，表明集胞藻在交替的垂直平面上分裂;（iv）MinCDE系统影响细胞的形态以及FtsZ结构的位置和形状;以及（v）MinD在涉及其C-末端两亲性螺旋但不涉及其ATP结合区域的过程中靶向细胞膜。最后，我们还表征了一种新的Z-相互作用蛋白，ZipN，其N-末端DnaJ结构域的Z-环的装饰是至关重要的，我们报告说，这个过程是独立的MinCDE。
Using a bacterial two-hybrid system and a combination of in vivo and in vitro assays that take advantage of the green fluorescent reporter protein (GFP), we have investigated the localization and the protein-protein interaction of several key components of the cytokinetic machinery of cyanobacteria (i.e. the progenitor of chloroplast). We demonstrate that (i) the ftsZ and zipN genes are essential for the viability of the model cyanobacterium Synechocystis sp. PCC 6803, whereas the minCDE cluster is dispensable for cell growth; (ii) the GTP-binding domain of FtsZ is crucial to FtsZ assembly into the septal ring at mid-cell; (iii) the Z-ring of deeply constricted daughter cells is oriented perpendicularly to the mother Z-ring, showing that Synechocystis divides in alternating perpendicular planes; (iv) the MinCDE system affects the morphology of the cell, as well as the position and the shape of FtsZ structures; and (v) MinD is targeted to cell membranes in a process involving its C-terminal amphipathic helix, but not its ATP-binding region. Finally, we have also characterized a novel Z-nteracting protein, ZipN, the N-terminal DnaJ domain of which is critical to the decoration of the Z-ring, and we report that this process is independent of MinCDE.