Substituted Aromatic-Facilitated Dissemination of Mobile Antibiotic Resistance Genes via an Antihydrolysis Mechanism Across an Extracellular Polymeric Substance Permeable Barrier
Substituted Aromatic-Facilitated Dissemination of Mobile Antibiotic Resistance Genes via an Antihydrolysis Mechanism Across an Extracellular Polymeric Substance Permeable Barrier
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通过抗水解机制替代芳香族促进移动 ARG 跨 EPS 渗透屏障的传播
DOI：
10.1021/acs.est.8b05750
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发表时间：
2019
影响因子：
11.4
通讯作者：
Wang Yijin
中科院分区：
文献类型：
--
作者：
Shou Weijun;Kang Fuxing;Huang Shuhan;Yan Chunyao;Zhou Jiaxin;Wang Yijin
环境系统中的移动的抗生素抗性基因（ARGs）可能对公众健康构成威胁。共存的取代芳香族污染物可能有助于ARG穿过胞外聚合物（EPS）渗透屏障进入细胞内部，促进ARG播散，但其机制尚不清楚。在这里，我们证明了一个特定的抗水解机制的移动的质粒在细胞外基质中作出了更大的贡献，这种促进传播。具体而言，荧光微量滴定与Tb 3+标记的pUC 19质粒被用来研究取代的芳香族质粒复合物的形成与ARG传播。操纵theendA基因和EPS证实，这些形成复合物拮抗EPS介导的水解的质粒。傅里叶变换红外光谱（FTIR）、X射线光电子能谱（XPS）和计算化学表明，取代基改变了芳香分子的极性，使1，3-二氯苯的6-位碳原子和不稳定的质子间苯二胺、苯胺和2-萘酚的（− NH 2/-OH）主要通过氢键与磷酸根的去质子化羟基（P-O···H-C/N/O）相互作用。ARG传播，关联常数和键能之间的线性相关性突出了ARG增殖的功能模板的组合的定量依赖性byd-ribose-phosphate。
Mobile antibiotic resistance genes (ARGs) in environmental systems may pose a threat to public health. The coexisting substituted aromatic pollutants may help the ARGs cross the extracellular polymeric substance (EPS) permeable barrier into the interior of cells, facilitating ARG dissemination, but the mechanism is still unknown. Here, we demonstrated that a specific antihydrolysis mechanism of mobile plasmid in the extracellular matrix makes a greater contribution to this facilitated dissemination. Specifically, fluorescence microtitration with a Tb3+-labeled pUC19 plasmid was used to study the formation of substituted aromatic–plasmid complexes associated with ARG dissemination. Manipulations of theendAgene and an EPS confirmed that these forming complexes antagonize the EPS-mediated hydrolysis of the plasmid. Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), and computational chemistry demonstrated that substituents alter the polarity of aromatic molecules, making the carbon at the 6-position of 1,3-dichlorobenzene as well as the labile protons (−NH2/–OH) ofm-phenylenediamine, aniline, and 2-naphthol interact with the deprotonated hydroxy group of the phosphate (P–O···H–C/N/O), mainly via hydrogen bonds. Linear correlations among ARG disseminations, association constants, and bonding energies highlight the quantitative dependency of ARG proliferation on a combination of functionalities templated byd-ribose–phosphate.